Enzymatic direct electron transfer at ascorbate oxidase-modified gold electrode prepared by one-step galvanostatic method

Bhushan Patil, Shigenori Fujikawa, Takeyoshi Okajima, Takeo Ohsaka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A one-step enzyme immobilization by galvanostatic method at gold electrode was applied to achieve direct electron transfer (DET) of ascorbate oxidase from Acremonium sp. (ASOM). The enzyme immobilization was optimized and ASOM was successfully deposited on the gold electrode surface, typically by flowing the oxidation current of 6 μA corresponding to oxygen evolution for 4 min, which results in a decrease in the local pH in the vicinity of the electrode surface and the precipitation of ASOM on the electrode surface due to the neutralization of its surface charge. A well-defined redox wave with a formal potential of 242 mV (vs. Ag|AgCl|KCl (sat)) was observed in 5.0 mM phosphate buffer solution (pH 7.0), confirming DET between ASOM and gold electrode surface.

Original languageEnglish
Pages (from-to)5012-5019
Number of pages8
JournalInternational Journal of Electrochemical Science
Volume7
Issue number6
Publication statusPublished - Jun 29 2012
Externally publishedYes

Fingerprint

Ascorbate Oxidase
Gold
Electrodes
Electrons
Enzyme immobilization
Surface charge
Buffers
Phosphates
Oxidoreductases
Oxygen
Oxidation

All Science Journal Classification (ASJC) codes

  • Electrochemistry

Cite this

Enzymatic direct electron transfer at ascorbate oxidase-modified gold electrode prepared by one-step galvanostatic method. / Patil, Bhushan; Fujikawa, Shigenori; Okajima, Takeyoshi; Ohsaka, Takeo.

In: International Journal of Electrochemical Science, Vol. 7, No. 6, 29.06.2012, p. 5012-5019.

Research output: Contribution to journalArticle

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