Enzymatic method for assaying uric acid in serum with a new tetrazolium salt produces water-soluble formazan dye

Yuzo Kayamori, Yoshiaki Katayama, Tatsuo Matsuyama, Takeyoshi Urata

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Objectives: To apply an enzymatic method for assaying uric acid in serum based on the uricase (EC 1.7.3.3)-catalase (EC 1.11.1.6)formaldehyde dehydrogenase (FADH, EC 1.2.1.46) coupled with the new tetrazolium salt producing a water-soluble formazan dye as an indicator system. Unlike the traditional tetrazolium salts, e.g., iodonitrotetrazolium (INT) and nitrotetrazolium blue (NTB), the corresponding formazan dye produced did not absorb to the test tube and the reaction cells of the analyzer. Moreover, this formazan dye had a higher water solubility and more sensitivity. Design and Methods: A two electron reduction of tetrazolium salt with NADH, produced by the uricase-catalase-FADH reaction, was mediated by an electron carrier, 1-methoxy PMS. The generation of formazan, monitored at 440 nm, was proportional to the concentration of uric acid in serum. The sensitivity of this method was about 1.5 times that of the peroxidase-TOOS [N-ethyl-N-(2- hydroxy-3-sulfopropyl)-m-toluidine] method. The assay was evaluated with TBA- 80FR. NEO biochemical analyzer. The average within-run and between-day imprecision (CV) were 0.75-2.44% and 3.20-3.33%, respectively. Results: Results of the proposed method (y) correlated well with those determined by the conventional chromogen method (x): y = 0.970 x - 0.018 mmol/L (Sy l x = 0.019 mmol/L, r = 0.993, n = 67). Conclusions: We also present data showing that the method is rapid, relatively free of interference, and amenable to automation.

Original languageEnglish
Pages (from-to)595-599
Number of pages5
JournalClinical Biochemistry
Volume30
Issue number8
DOIs
Publication statusPublished - Dec 1 1997

Fingerprint

Tetrazolium Salts
Formazans
Saline water
Uric Acid
Coloring Agents
Urate Oxidase
glutathione-independent formaldehyde dehydrogenase
Water
Serum
Catalase
Nitroblue Tetrazolium
Electrons
NAD
Peroxidase
Assays
Automation
Solubility

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Enzymatic method for assaying uric acid in serum with a new tetrazolium salt produces water-soluble formazan dye. / Kayamori, Yuzo; Katayama, Yoshiaki; Matsuyama, Tatsuo; Urata, Takeyoshi.

In: Clinical Biochemistry, Vol. 30, No. 8, 01.12.1997, p. 595-599.

Research output: Contribution to journalArticle

Kayamori, Yuzo ; Katayama, Yoshiaki ; Matsuyama, Tatsuo ; Urata, Takeyoshi. / Enzymatic method for assaying uric acid in serum with a new tetrazolium salt produces water-soluble formazan dye. In: Clinical Biochemistry. 1997 ; Vol. 30, No. 8. pp. 595-599.
@article{24ff31fa1cad44c684055846b087f94d,
title = "Enzymatic method for assaying uric acid in serum with a new tetrazolium salt produces water-soluble formazan dye",
abstract = "Objectives: To apply an enzymatic method for assaying uric acid in serum based on the uricase (EC 1.7.3.3)-catalase (EC 1.11.1.6)formaldehyde dehydrogenase (FADH, EC 1.2.1.46) coupled with the new tetrazolium salt producing a water-soluble formazan dye as an indicator system. Unlike the traditional tetrazolium salts, e.g., iodonitrotetrazolium (INT) and nitrotetrazolium blue (NTB), the corresponding formazan dye produced did not absorb to the test tube and the reaction cells of the analyzer. Moreover, this formazan dye had a higher water solubility and more sensitivity. Design and Methods: A two electron reduction of tetrazolium salt with NADH, produced by the uricase-catalase-FADH reaction, was mediated by an electron carrier, 1-methoxy PMS. The generation of formazan, monitored at 440 nm, was proportional to the concentration of uric acid in serum. The sensitivity of this method was about 1.5 times that of the peroxidase-TOOS [N-ethyl-N-(2- hydroxy-3-sulfopropyl)-m-toluidine] method. The assay was evaluated with TBA- 80FR. NEO biochemical analyzer. The average within-run and between-day imprecision (CV) were 0.75-2.44{\%} and 3.20-3.33{\%}, respectively. Results: Results of the proposed method (y) correlated well with those determined by the conventional chromogen method (x): y = 0.970 x - 0.018 mmol/L (Sy l x = 0.019 mmol/L, r = 0.993, n = 67). Conclusions: We also present data showing that the method is rapid, relatively free of interference, and amenable to automation.",
author = "Yuzo Kayamori and Yoshiaki Katayama and Tatsuo Matsuyama and Takeyoshi Urata",
year = "1997",
month = "12",
day = "1",
doi = "10.1016/S0009-9120(97)00118-5",
language = "English",
volume = "30",
pages = "595--599",
journal = "Clinical Biochemistry",
issn = "0009-9120",
publisher = "Elsevier Inc.",
number = "8",

}

TY - JOUR

T1 - Enzymatic method for assaying uric acid in serum with a new tetrazolium salt produces water-soluble formazan dye

AU - Kayamori, Yuzo

AU - Katayama, Yoshiaki

AU - Matsuyama, Tatsuo

AU - Urata, Takeyoshi

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Objectives: To apply an enzymatic method for assaying uric acid in serum based on the uricase (EC 1.7.3.3)-catalase (EC 1.11.1.6)formaldehyde dehydrogenase (FADH, EC 1.2.1.46) coupled with the new tetrazolium salt producing a water-soluble formazan dye as an indicator system. Unlike the traditional tetrazolium salts, e.g., iodonitrotetrazolium (INT) and nitrotetrazolium blue (NTB), the corresponding formazan dye produced did not absorb to the test tube and the reaction cells of the analyzer. Moreover, this formazan dye had a higher water solubility and more sensitivity. Design and Methods: A two electron reduction of tetrazolium salt with NADH, produced by the uricase-catalase-FADH reaction, was mediated by an electron carrier, 1-methoxy PMS. The generation of formazan, monitored at 440 nm, was proportional to the concentration of uric acid in serum. The sensitivity of this method was about 1.5 times that of the peroxidase-TOOS [N-ethyl-N-(2- hydroxy-3-sulfopropyl)-m-toluidine] method. The assay was evaluated with TBA- 80FR. NEO biochemical analyzer. The average within-run and between-day imprecision (CV) were 0.75-2.44% and 3.20-3.33%, respectively. Results: Results of the proposed method (y) correlated well with those determined by the conventional chromogen method (x): y = 0.970 x - 0.018 mmol/L (Sy l x = 0.019 mmol/L, r = 0.993, n = 67). Conclusions: We also present data showing that the method is rapid, relatively free of interference, and amenable to automation.

AB - Objectives: To apply an enzymatic method for assaying uric acid in serum based on the uricase (EC 1.7.3.3)-catalase (EC 1.11.1.6)formaldehyde dehydrogenase (FADH, EC 1.2.1.46) coupled with the new tetrazolium salt producing a water-soluble formazan dye as an indicator system. Unlike the traditional tetrazolium salts, e.g., iodonitrotetrazolium (INT) and nitrotetrazolium blue (NTB), the corresponding formazan dye produced did not absorb to the test tube and the reaction cells of the analyzer. Moreover, this formazan dye had a higher water solubility and more sensitivity. Design and Methods: A two electron reduction of tetrazolium salt with NADH, produced by the uricase-catalase-FADH reaction, was mediated by an electron carrier, 1-methoxy PMS. The generation of formazan, monitored at 440 nm, was proportional to the concentration of uric acid in serum. The sensitivity of this method was about 1.5 times that of the peroxidase-TOOS [N-ethyl-N-(2- hydroxy-3-sulfopropyl)-m-toluidine] method. The assay was evaluated with TBA- 80FR. NEO biochemical analyzer. The average within-run and between-day imprecision (CV) were 0.75-2.44% and 3.20-3.33%, respectively. Results: Results of the proposed method (y) correlated well with those determined by the conventional chromogen method (x): y = 0.970 x - 0.018 mmol/L (Sy l x = 0.019 mmol/L, r = 0.993, n = 67). Conclusions: We also present data showing that the method is rapid, relatively free of interference, and amenable to automation.

UR - http://www.scopus.com/inward/record.url?scp=0031422756&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031422756&partnerID=8YFLogxK

U2 - 10.1016/S0009-9120(97)00118-5

DO - 10.1016/S0009-9120(97)00118-5

M3 - Article

C2 - 9455611

AN - SCOPUS:0031422756

VL - 30

SP - 595

EP - 599

JO - Clinical Biochemistry

JF - Clinical Biochemistry

SN - 0009-9120

IS - 8

ER -