TY - JOUR
T1 - Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae
AU - Takegawa, Kaoru
AU - Yamaguchi, S.
AU - Kondo, A.
AU - Kato, I.
AU - Iwahara, S.
PY - 1991
Y1 - 1991
N2 - The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.
AB - The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.
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M3 - Article
C2 - 1804102
AN - SCOPUS:0026354070
VL - 25
SP - 829
EP - 835
JO - Biochemistry and Molecular Biology International
JF - Biochemistry and Molecular Biology International
SN - 1521-6543
IS - 5
ER -