Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Kaoru Takegawa, S. Yamaguchi, A. Kondo, I. Kato, S. Iwahara

Research output: Contribution to journalArticlepeer-review

45 Citations (Scopus)

Abstract

The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

Original languageEnglish
Pages (from-to)829-835
Number of pages7
JournalBiochemistry International
Volume25
Issue number5
Publication statusPublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry

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