Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Kaoru Takegawa; S. Yamaguchi; A. Kondo; I. Kato; S. Iwahara

Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Kaoru Takegawa, S. Yamaguchi, A. Kondo, I. Kato, S. Iwahara

Research output: Contribution to journal › Article › peer-review

Abstract

The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)₆(GlcNAc)₂Asn was used as a substrate for the transglycosylation, (Man)₆GlcNAc-Glc, (Man)₆GlcNAc-Man, (Man)₆GlcNAc-chitobiose, and (Man)₆GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

Original language	English
Pages (from-to)	829-835
Number of pages	7
Journal	Biochemistry International
Volume	25
Issue number	5
Publication status	Published - 1991
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biochemistry

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title = "Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae",

abstract = "The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.",

author = "Kaoru Takegawa and S. Yamaguchi and A. Kondo and I. Kato and S. Iwahara",

year = "1991",

language = "English",

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AU - Takegawa, Kaoru

AU - Yamaguchi, S.

AU - Kondo, A.

AU - Kato, I.

AU - Iwahara, S.

PY - 1991

Y1 - 1991

N2 - The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

AB - The transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae was used for the enzymatic synthesis of novel oligosaccharides. When (Man)6(GlcNAc)2Asn was used as a substrate for the transglycosylation, (Man)6GlcNAc-Glc, (Man)6GlcNAc-Man, (Man)6GlcNAc-chitobiose, and (Man)6GlcNAc-gentiobiose were synthesized. Their structures were identified by HPLC, ion spray mass spectrometry, and digestion with glycosidases Endo-β-N-acetylglucosaminidases hydrolyzed the pyridylamino derivatives of these oligosaccharides.

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Enzymatic synthesis of novel oligosaccharides by use of transglycosylation activity of endo-β-N-acetylglucosaminidase from Arthrobacter protophormiae

Abstract

All Science Journal Classification (ASJC) codes

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