Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui, Koki Motoyama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journalArticle

Abstract

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

Original languageEnglish
Pages (from-to)3237-3240
Number of pages4
JournalRSC Advances
Volume3
Issue number10
DOIs
Publication statusPublished - Mar 14 2013

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Protocatechuate-3,4-Dioxygenase
DNA
Enzymes
Molecules
Microscopes
Physical properties
Acids
protocatechuic acid

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules. / Yasui, Takao; Motoyama, Koki; Kaji, Noritada; Tokeshi, Manabu; Baba, Yoshinobu.

In: RSC Advances, Vol. 3, No. 10, 14.03.2013, p. 3237-3240.

Research output: Contribution to journalArticle

Yasui, Takao ; Motoyama, Koki ; Kaji, Noritada ; Tokeshi, Manabu ; Baba, Yoshinobu. / Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules. In: RSC Advances. 2013 ; Vol. 3, No. 10. pp. 3237-3240.
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