Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui; Koki Motoyama; Noritada Kaji; Manabu Tokeshi; Yoshinobu Baba

doi:10.1039/c3ra22999h

Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Takao Yasui, Koki Motoyama, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

Original language	English
Pages (from-to)	3237-3240
Number of pages	4
Journal	RSC Advances
Volume	3
Issue number	10
DOIs	https://doi.org/10.1039/c3ra22999h
Publication status	Published - Mar 14 2013
Externally published	Yes

All Science Journal Classification (ASJC) codes

Chemistry(all)
Chemical Engineering(all)

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abstract = "The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.",

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AU - Kaji, Noritada

AU - Tokeshi, Manabu

AU - Baba, Yoshinobu

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AB - The technical development of long-term fluorescent observation of single DNA molecules is central to fields ranging from molecular biological detection to understanding the physical properties of them under a microscope. Here, we address this challenge using protocatechuic acid and protocatechuate-3,4- dioxygenase (PADase) and demonstrate fluorescent lifetimes of dyed single DNA molecules of 150-180 s, three times longer than those without any treatments.

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Enzyme-catalysed reaction for long-term fluorescent observation of single DNA molecules

Abstract

All Science Journal Classification (ASJC) codes

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