Enzyme encapsulation in microparticles composed of polymerized ionic liquids for highly active and reusable biocatalysts

Kazunori Nakashima, Noriho Kamiya, Daisuke Koda, Tatsuo Maruyama, Masahiro Goto

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Horseradish peroxidase (HRP) is encapsulated in polymerized ionic liquid microparticles (pIL-MP), which are prepared by polymerization of 1-vinyl-3-ethylimidazolium bromide in the presence of the crosslinker N,N′-methylenebis(acrylamide) in a concentrated water-in-oil (W/O) emulsion. pIL-MP encapsulating HRP chemically-modified with comb-shaped polyethylene glycol (PM13-HRP) exhibit excellent activity for guaiacol oxidation in an aqueous solution. The PM13-HRP in pIL-MP shows more than 2-fold higher activity than that of the enzyme encapsulated in a polyacrylamide microparticle. The catalytic activity declines with an increase in the crosslinker concentration of the pIL-MP, probably due to suppression of substrate diffusion. The activity of PM13-HRP in pIL-MP depends on the external environment of the gel (i.e. pH and temperature). The pIL-MP are easily recovered from the reaction mixture by centrifugation, which makes it possible to recycle the biocatalyst for repeated oxidation reactions.

Original languageEnglish
Pages (from-to)2353-2358
Number of pages6
JournalOrganic and Biomolecular Chemistry
Volume7
Issue number11
DOIs
Publication statusPublished - Jun 1 2009

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All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Biochemistry

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