Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

Osamu Morinaga; Hiroyuki Tanaka; Yukihiro Shoyama

doi:10.1080/00032710500476979

Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

Pharmaceutical Health Care and Sciences

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ²) of 0.995.

Original language	English
Pages (from-to)	287-296
Number of pages	10
Journal	Analytical Letters
Volume	39
Issue number	2
DOIs	https://doi.org/10.1080/00032710500476979
Publication status	Published - Jan 2006

All Science Journal Classification (ASJC) codes

Analytical Chemistry
Biochemistry
Spectroscopy
Clinical Biochemistry
Biochemistry, medical
Electrochemistry

Access to Document

10.1080/00032710500476979

Cite this

@article{55c4cb034ce34c12be1f0fbc30f8374d,

title = "Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng",

abstract = "Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ2) of 0.995.",

author = "Osamu Morinaga and Hiroyuki Tanaka and Yukihiro Shoyama",

note = "Funding Information: Received 2 September 2005; accepted 21 October 2005 This research was supported by Japan Science and Technology Agency. Address correspondence to Yukihiro Shoyama, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.",

year = "2006",

month = jan,

doi = "10.1080/00032710500476979",

language = "English",

volume = "39",

pages = "287--296",

journal = "Analytical Letters",

issn = "0003-2719",

publisher = "Taylor and Francis Ltd.",

number = "2",

}

TY - JOUR

T1 - Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

AU - Morinaga, Osamu

AU - Tanaka, Hiroyuki

AU - Shoyama, Yukihiro

N1 - Funding Information: Received 2 September 2005; accepted 21 October 2005 This research was supported by Japan Science and Technology Agency. Address correspondence to Yukihiro Shoyama, Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.

PY - 2006/1

Y1 - 2006/1

N2 - Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ2) of 0.995.

AB - Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ2) of 0.995.

UR - http://www.scopus.com/inward/record.url?scp=33644888855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644888855&partnerID=8YFLogxK

U2 - 10.1080/00032710500476979

DO - 10.1080/00032710500476979

M3 - Article

AN - SCOPUS:33644888855

SN - 0003-2719

VL - 39

SP - 287

EP - 296

JO - Analytical Letters

JF - Analytical Letters

IS - 2

ER -

Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this