Enzyme-linked immunosorbent assay for the determination of total ginsenosides in ginseng

Osamu Morinaga, Hiroyuki Tanaka, Yukihiro Shoyama

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11 Citations (Scopus)

Abstract

Hybridoma secreting a monoclonal antibody (MAb) against ginsenosides was produced by fusing splenocytes from a mouse immunized against a ginsenoside Re-bovine serum albumin (G-Re-BSA) conjugate with myeloma cell line SP2/0-Ag14. A MAb-4G10 had a wide cross-reaction with 20(5)-protopanaxadiol and 20(S)-protopanaxatriol type ginsenosides. An enzyme-linked immunosorbent assay (ELISA) that had an effective measuring range of 20-400 ng/mL for total ginsenosides when G-Re was used as a standard. Total ginsenosides concentration in ginseng were determined by ELISA and high-performance liquid chromatography (HPLC) in a good agreement together, and the least-squares fit had a coefficient of determination (γ2) of 0.995.

Original languageEnglish
Pages (from-to)287-296
Number of pages10
JournalAnalytical Letters
Volume39
Issue number2
DOIs
Publication statusPublished - Jan 1 2006

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All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Spectroscopy
  • Clinical Biochemistry
  • Biochemistry, medical
  • Electrochemistry

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