Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies

Benyakan Pongkitwitoon, Seiichi Sakamoto, Hiroyuki Tanaka, Ryota Tsuchihashi, Junei Kinjo, Satoshi Morimoto, Waraporn Putalun

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Pueraria candollei (White Kwao Khuer) is a medicinal plant containing puerarin, daidzin, genistin, daidzein, and genistein as major isoflavonoids used for its rejuvenating and estrogenic effects. In order to analyze these compounds, a single enzyme-linked immunosorbent assay (ELISA) for total isoflavonoids was developed using anti-puerarin and anti-daidzin polyclonal antibodies (PAbs). The range for calibration of isoflavonoids by ELISA was 0.056.25g/mL. Total isoflavonoid concentrations in P. candollei samples determined by the newly developed assay system showed good agreement with those analyzed by HPLC. Based on validation analysis, this analytical method by ELISA is a precise, accurate, and sensitive method for the determination of total isoflavonoids in P. candollei.

Original languageEnglish
Pages (from-to)831-836
Number of pages6
JournalPlanta medica
Volume76
Issue number8
DOIs
Publication statusPublished - Jun 4 2010

Fingerprint

Pueraria
Immunosorbents
Assays
Enzyme-Linked Immunosorbent Assay
Antibodies
Enzymes
Genistein
Medicinal Plants
Calibration
Estrogens
High Pressure Liquid Chromatography
puerarin
daidzin

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine
  • Organic Chemistry

Cite this

Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies. / Pongkitwitoon, Benyakan; Sakamoto, Seiichi; Tanaka, Hiroyuki; Tsuchihashi, Ryota; Kinjo, Junei; Morimoto, Satoshi; Putalun, Waraporn.

In: Planta medica, Vol. 76, No. 8, 04.06.2010, p. 831-836.

Research output: Contribution to journalArticle

Pongkitwitoon, Benyakan ; Sakamoto, Seiichi ; Tanaka, Hiroyuki ; Tsuchihashi, Ryota ; Kinjo, Junei ; Morimoto, Satoshi ; Putalun, Waraporn. / Enzyme-linked immunosorbent assay for total isoflavonoids in pueraria candollei using anti-puerarin and anti-daidzin polyclonal antibodies. In: Planta medica. 2010 ; Vol. 76, No. 8. pp. 831-836.
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AU - Tanaka, Hiroyuki

AU - Tsuchihashi, Ryota

AU - Kinjo, Junei

AU - Morimoto, Satoshi

AU - Putalun, Waraporn

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