Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies

Osamu Morinaga, Takuhiro Uto, Seiichi Sakamoto, Hiroyuki Tanaka, Yukihiro Shoyama

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.

Original languageEnglish
Pages (from-to)28-31
Number of pages4
JournalFitoterapia
Volume80
Issue number1
DOIs
Publication statusPublished - Jan 2009

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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