TY - JOUR
T1 - Enzyme-linked immunosorbent assay for total sennosides using anti-sennside A and anti-sennoside B monoclonal antibodies
AU - Morinaga, Osamu
AU - Uto, Takuhiro
AU - Sakamoto, Seiichi
AU - Tanaka, Hiroyuki
AU - Shoyama, Yukihiro
N1 - Funding Information:
This research was supported in part by a Grant-in-Aid for Scientific Research, from the Ministry of Science and Culture of Japan, the research fund of Japan Society for the Promotion of Science (JSPS's Asian CORE Program), the research aid of Japan Science and Technology Agency (excavation of seeds) and Takeda Science Foundation.
PY - 2009/1
Y1 - 2009/1
N2 - Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
AB - Total sennosides concentration is a very important factor when rhubarb and senna will be used as crude drugs. However, one-step analytical technique for total sennosides has not been reported except HPLC. An enzyme-linked immunosorbent assay (ELISA) for total sennosides concentration by using the combination of anti-sennoside A (SA) and anti-sennoside B (SB) monoclonal antibodies (MAbs) in a single assay has been investigated. Total sennosides concentration in rhubarb and senna samples determined by newly developed assay system showed good agreement with those analyzed by ELISA using anti-SA MAb and anti-SB MAb, respectively.
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U2 - 10.1016/j.fitote.2008.09.004
DO - 10.1016/j.fitote.2008.09.004
M3 - Article
C2 - 18926890
AN - SCOPUS:58149099497
SN - 0367-326X
VL - 80
SP - 28
EP - 31
JO - Fitoterapia
JF - Fitoterapia
IS - 1
ER -