Enzyme-mediated protein refolding

Jun Okada, Tatsuo Maruyama, Konomi Motomura, Kimiko Kuroki, Katsumi Maenaka, Masafumi Sakono, Masahiro Goto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

Original languageEnglish
Pages (from-to)7197-7199
Number of pages3
JournalChemical Communications
Issue number46
DOIs
Publication statusPublished - Nov 27 2009

Fingerprint

Urease
Enzymes
Proteins
Urea
Dilution

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

Cite this

Okada, J., Maruyama, T., Motomura, K., Kuroki, K., Maenaka, K., Sakono, M., & Goto, M. (2009). Enzyme-mediated protein refolding. Chemical Communications, (46), 7197-7199. https://doi.org/10.1039/b916225a

Enzyme-mediated protein refolding. / Okada, Jun; Maruyama, Tatsuo; Motomura, Konomi; Kuroki, Kimiko; Maenaka, Katsumi; Sakono, Masafumi; Goto, Masahiro.

In: Chemical Communications, No. 46, 27.11.2009, p. 7197-7199.

Research output: Contribution to journalArticle

Okada, J, Maruyama, T, Motomura, K, Kuroki, K, Maenaka, K, Sakono, M & Goto, M 2009, 'Enzyme-mediated protein refolding', Chemical Communications, no. 46, pp. 7197-7199. https://doi.org/10.1039/b916225a
Okada J, Maruyama T, Motomura K, Kuroki K, Maenaka K, Sakono M et al. Enzyme-mediated protein refolding. Chemical Communications. 2009 Nov 27;(46):7197-7199. https://doi.org/10.1039/b916225a
Okada, Jun ; Maruyama, Tatsuo ; Motomura, Konomi ; Kuroki, Kimiko ; Maenaka, Katsumi ; Sakono, Masafumi ; Goto, Masahiro. / Enzyme-mediated protein refolding. In: Chemical Communications. 2009 ; No. 46. pp. 7197-7199.
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