Enzyme-mediated protein refolding

Jun Okada, Tatsuo Maruyama, Konomi Motomura, Kimiko Kuroki, Katsumi Maenaka, Masafumi Sakono, Masahiro Goto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

Original languageEnglish
Pages (from-to)7197-7199
Number of pages3
JournalChemical Communications
Issue number46
DOIs
Publication statusPublished - 2009

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Electronic, Optical and Magnetic Materials
  • Ceramics and Composites
  • Chemistry(all)
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Materials Chemistry

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  • Cite this

    Okada, J., Maruyama, T., Motomura, K., Kuroki, K., Maenaka, K., Sakono, M., & Goto, M. (2009). Enzyme-mediated protein refolding. Chemical Communications, (46), 7197-7199. https://doi.org/10.1039/b916225a