Enzyme-mediated protein refolding

Jun Okada, Tatsuo Maruyama, Konomi Motomura, Kimiko Kuroki, Katsumi Maenaka, Masafumi Sakono, Masahiro Goto

    Research output: Contribution to journalArticlepeer-review

    4 Citations (Scopus)

    Abstract

    We employed a urease-catalyzed reaction to gradually remove a high concentration of a chaotropic agent (urea) from a denatured protein solution and demonstrated that efficient protein refolding can be achieved by the urease-catalyzed reaction, without large-volume dilution.

    Original languageEnglish
    Pages (from-to)7197-7199
    Number of pages3
    JournalChemical Communications
    Issue number46
    DOIs
    Publication statusPublished - 2009

    All Science Journal Classification (ASJC) codes

    • Catalysis
    • Electronic, Optical and Magnetic Materials
    • Ceramics and Composites
    • Chemistry(all)
    • Surfaces, Coatings and Films
    • Metals and Alloys
    • Materials Chemistry

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