健康な看護学生から分離された黄色ブドウ球菌の疫学調査: エンテロトキシンおよびTSST-1産生とコアグラーゼ遺伝子型別(保健福祉部看護学科)

Translated title of the contribution: Epidemiological Study of Staphylococcus aureus Isolated from Healthy Student-Norses: Production of Enterotoxins and Toxic Shock Syndrome Toxin 1 and Coagulase Gene Typing

目野 郁子, 吉武 美佐子, 藤本 秀士

Research output: Contribution to journalArticle

Abstract

The production of staphylococcal enterotoxin (SE) A to D or toxic shock syndrome toxin 1 (TSST-1) and coagulase gene typing were examined in 91 Staphylococcus aureus (S. aureus) strains isolated from nares of healthy student-nurses. Of the 91 isolates, 43 strains (47.3%) produced at least one of the SEA to SED or TSST-1. In the toxin-producing strains, TSST-1 (TSST-1, TSST-1 + SEA, TSST-1 + SEC), SEA (SEA, SEA + SEE, TSST-1 + SEA), and SEE (SEE, SEA + SEE) were detected in relatively high incidence, 46.5%, 41.9% and 34.9%, respectively. Coagulase gene typing was performed by polymerase chain reaction (PCR) amplification of coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the PCR products. Among all of the isolates, there were 12 different RFLP patterns (A〜L). The 43 toxin-producing strains belonged to 8 patterns (A〜F) of the 12 patterns and pattern F (51.2%) was most frequently observed, followed by pattern B (20.9%). Pattern F was seen with most of the TSST-1- producing strains (TSST, TSST + SEA, TSST + SEC) and pattern B was observed in the SEB-producing strains.
Translated title of the contributionEpidemiological Study of Staphylococcus aureus Isolated from Healthy Student-Norses: Production of Enterotoxins and Toxic Shock Syndrome Toxin 1 and Coagulase Gene Typing
Original languageJapanese
Pages (from-to)9-14
Number of pages6
Journal西南女学院大学紀要
Volume9
Publication statusPublished - Feb 28 2005

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