Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells

Shuhei Yamada, Shuntaro Tsukamoto, Yuhui Huang, Akiko Makio, Motofumi Kumazoe, Shuya Yamashita, Hirofumi Tachibana

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Abstract

MicroRNAs (miRNAs) are non-coding RNAs involved in various biological processes by regulating their target genes. Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibits melanoma tumor growth by activating 67-kDa laminin receptor (67LR) signaling. To examine the effect of EGCG on miRNA expression in melanoma cells, we performed miRNA microarray analysis. We showed that EGCG up-regulated miRNA-let-7b expression through 67LR in melanoma cells. The EGCG-induced up-regulation of let-7b led to down-regulation of high mobility group A2 (HMGA2), a target gene related to tumor progression. 67LR-dependent cAMP/protein kinase A (PKA)/protein phosphatase 2A (PP2A) signaling pathway activation was involved in the up-regulation of let-7b expression induced by EGCG. These findings provide a basis for understanding the mechanism of miRNA regulation by EGCG.

Original languageEnglish
Article number19225
JournalScientific reports
Volume6
DOIs
Publication statusPublished - Jan 12 2016

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Laminin Receptors
MicroRNAs
Melanoma
Up-Regulation
Biological Phenomena
Protein Phosphatase 2
Untranslated RNA
Polyphenols
Tea
Microarray Analysis
Cyclic AMP-Dependent Protein Kinases
varespladib methyl
Genes
epigallocatechin gallate
Neoplasms
Down-Regulation
Growth

All Science Journal Classification (ASJC) codes

  • General

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Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells. / Yamada, Shuhei; Tsukamoto, Shuntaro; Huang, Yuhui; Makio, Akiko; Kumazoe, Motofumi; Yamashita, Shuya; Tachibana, Hirofumi.

In: Scientific reports, Vol. 6, 19225, 12.01.2016.

Research output: Contribution to journalArticle

Yamada, Shuhei ; Tsukamoto, Shuntaro ; Huang, Yuhui ; Makio, Akiko ; Kumazoe, Motofumi ; Yamashita, Shuya ; Tachibana, Hirofumi. / Epigallocatechin-3-O-gallate up-regulates microRNA-let-7b expression by activating 67-kDa laminin receptor signaling in melanoma cells. In: Scientific reports. 2016 ; Vol. 6.
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AU - Makio, Akiko

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