Epigallocatechin gallate promotes the vasorelaxation power of the antiatherosclerotic dipeptide trp-his in contracted rat aorta

Mitsuru Tanaka, Jian Zhao, Aki Suyama, Toshiro Matsui

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study was to demonstrate the enhancement of the vasorelaxation power of the antiatherosclerotic voltage-dependent L-type Ca 2+ channel (VDCC)-blocking peptide Trp-His by epigallocatechin gallate (EGCg). We found that 300 μM EGCg dramatically enhanced the magnitude of Trp-His-induced vasorelaxation by a factor of >6 (EC50 of Trp-His: EGCg(-), 2.80 ± 0.05 mM; EGCg(+), 0.45 ± 0.04 mM) in phenylephrine-contracted rat aorta. The enhancing effect of EGCg was completely abolished in endothelium-removed aorta and high K+-contracted aorta. The enhancement of Trp-His-induced vasorelaxation by EGCg was significantly diminished by either NG-monomethyl-l-arginine acetate (NO synthase (NOS) inhibitor) or 1-H-[1,2,4]oxadiazolo[4,3]quinoxalin-1-one (soluble guanylyl cyclase inhibitor), together with the enhancement of NOS activity by EGCg. These results indicate that the enhancing effect of EGCg in Trp-His-induced vasorelaxation may be involved in the activation of NO/cGMP pathway.

Original languageEnglish
Pages (from-to)9048-9054
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume60
Issue number36
DOIs
Publication statusPublished - Sep 12 2012

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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