TY - JOUR
T1 - Epigenome mapping of human normal purified hepatocytes
T2 - Personal epigenome variation and genome-epigenome correlation
AU - Arai, Eri
AU - Miura, Fumihito
AU - Totoki, Yasushi
AU - Yamashita, Satoshi
AU - Tian, Ying
AU - Gotoh, Masahiro
AU - Ojima, Hidenori
AU - Nakagawa, Hiroyuki
AU - Takahashi, Yoriko
AU - Nakamura, Hiromi
AU - Hama, Natsuko
AU - Kato, Mamoru
AU - Kimura, Hiroshi
AU - Suzuki, Yutaka
AU - Ito, Takashi
AU - Shibata, Tatsuhiro
AU - Kanai, Yae
N1 - Funding Information:
This work was supported by AMED-CREST, Japan Agency for Medical Research and Development (16gm0510003h0006). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript.
Publisher Copyright:
© 2018 Yae Kanai.
PY - 2018/7
Y1 - 2018/7
N2 - Aim: The aim of this study was to reveal the epigenome landscape of human normal hepatocytes. Materials & methods: Cells purified from partial hepatectomy specimens of Japanese patients were subjected to whole-genome bisulfite sequencing using postbisulfite adaptor tagging, chromatin immunoprecipitation sequencing, RNA sequencing and whole-genome sequencing. Results: CHG and CHH methylations were inversely associated with gene expression. Histone modification profiles of personal differentially methylated regions (pDMRs) differed considerably among samples. pDMRs were observed around the transcription start sites of genes whose expression is reportedly regulated by CpG methylation. pDMRs were frequently observed in the vicinity of single-nucleotide variations and insertions/deletions. Conclusion: Genetic variations may induce epigenetic variations, generating individual differences in the phenotypes of normal hepatocytes through variations in expression.
AB - Aim: The aim of this study was to reveal the epigenome landscape of human normal hepatocytes. Materials & methods: Cells purified from partial hepatectomy specimens of Japanese patients were subjected to whole-genome bisulfite sequencing using postbisulfite adaptor tagging, chromatin immunoprecipitation sequencing, RNA sequencing and whole-genome sequencing. Results: CHG and CHH methylations were inversely associated with gene expression. Histone modification profiles of personal differentially methylated regions (pDMRs) differed considerably among samples. pDMRs were observed around the transcription start sites of genes whose expression is reportedly regulated by CpG methylation. pDMRs were frequently observed in the vicinity of single-nucleotide variations and insertions/deletions. Conclusion: Genetic variations may induce epigenetic variations, generating individual differences in the phenotypes of normal hepatocytes through variations in expression.
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U2 - 10.2217/epi-2017-0111
DO - 10.2217/epi-2017-0111
M3 - Article
C2 - 29972026
AN - SCOPUS:85049835936
SN - 1750-1911
VL - 10
SP - 955
EP - 979
JO - Epigenomics
JF - Epigenomics
IS - 7
ER -
