Epimorphin acts extracellularly to promote cell sorting and aggregation during the condensation of vertebral cartilage

Yumiko Oka, Yuki Sato, Hokari Tsuda, Kazunori Hanaoka, Yohei Hirai, Yoshiko Takahashi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Formation of vertebrae occurs via endochondral ossification, a process involving condensation of precartilaginous cells. Here, we provide the first molecular evidence of mechanism that underlies initiation of this process by showing that the extracellular factor, Epimorphin, plays a role during early steps in vertebral cartilage condensation. Epimorphin mRNA is predominantly localized in the vertebral primordium. When provided exogenously in ovo, it causes precocious differentiation of chondrocytes, resulting in the formation of supernumerary vertebral cartilage in chicken embryos. To further analyze its mode of action, we used an in vitro co-culture system in which labeled 10T1/2 or sclerotomal prechondrogenic cells were co-cultured with unlabeled Epimorphin-producing cells. In the presence of Epimorphin, the labeled cells formed tightly packed aggregates, and sclerotomal cells displayed augmented accumulation of NCAM and other early markers of chondrocyte differentiation. Finally, we found that the Epimorphin expression is initiated during vertebrogenesis by Sonic hedgehog from the notochord mediated by Sox 9. We present a model in which successive action of Epimorphin in recruiting and stacking sclerotomal cells leads to a sequential elongation of a vertebral primordium.

Original languageEnglish
Pages (from-to)25-37
Number of pages13
JournalDevelopmental Biology
Volume291
Issue number1
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

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Cell Aggregation
Cartilage
Chondrocytes
Notochord
Neural Cell Adhesion Molecules
Hedgehogs
Differentiation Antigens
Coculture Techniques
Osteogenesis
Chickens
Spine
Embryonic Structures
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Epimorphin acts extracellularly to promote cell sorting and aggregation during the condensation of vertebral cartilage. / Oka, Yumiko; Sato, Yuki; Tsuda, Hokari; Hanaoka, Kazunori; Hirai, Yohei; Takahashi, Yoshiko.

In: Developmental Biology, Vol. 291, No. 1, 01.03.2006, p. 25-37.

Research output: Contribution to journalArticle

Oka, Yumiko ; Sato, Yuki ; Tsuda, Hokari ; Hanaoka, Kazunori ; Hirai, Yohei ; Takahashi, Yoshiko. / Epimorphin acts extracellularly to promote cell sorting and aggregation during the condensation of vertebral cartilage. In: Developmental Biology. 2006 ; Vol. 291, No. 1. pp. 25-37.
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