Epithelial Paradox: Clinical Significance of Coexpression of E-cadherin and Vimentin With Regard to Invasion and Metastasis of Breast Cancer

Nami Yamashita, Eriko Tokunaga, Makoto Iimori, Yuka Inoue, Kimihiro Tanaka, Hiroyuki Kitao, Hiroshi Saeki, Eiji Oki, Yoshihiko Maehara

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Abstract

E-cadherin and vimentin are regarded as major conventional canonical markers of epithelial–mesenchymal transition. Both E-cadherin– and vimentin-positive tumors had the worst prognosis among all cases. Further, E-cadherin and vimentin protein is colocalized within the same tumor cells, suggesting the existence of an aggressive subpopulation in the primary tumor nest of breast cancer. Background: E-cadherin and vimentin are regarded as major conventional canonical markers of the epithelial–mesenchymal transition. It is commonly assumed that E-cadherin is uniformly lost during the process of epithelial–mesenchymal transition. Breast tumor cells typically invade as a cohesive multicellular unit in a process called collective invasion. The aim of this study was to reveal the clinical importance of the expression pattern of E-cadherin and vimentin in breast cancer. Methods: E-cadherin and vimentin protein expression was evaluated by immunohistochemistry in 176 invasive breast cancer samples. Among these, E-cadherin and vimentin expression were evaluated in the set of primary site and metastatic lymph nodes in 65 cases. In addition, E-cadherin and vimentin expression were analyzed by confocal laser scanning microscopy to see E-cadherin and vimentin localization in the breast cancer cells. Results: Both at the primary site and metastatic lymph nodes, both E-cadherin– and vimentin-positive tumors had the worst disease-free and overall survival among all cases. In addition, E-cadherin and vimentin protein is colocalized within the same tumor cells in a human breast cancer specimen. Conclusion: Our present data suggest the existence of an aggressive subpopulation in the primary tumor nest of breast cancer.

Original languageEnglish
Pages (from-to)e1003-e1009
JournalClinical Breast Cancer
Volume18
Issue number5
DOIs
Publication statusPublished - Oct 1 2018

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Vimentin
Cadherins
Breast Neoplasms
Neoplasm Metastasis
Neoplasms
Lymph Nodes
Proteins
Confocal Microscopy
Disease-Free Survival
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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Epithelial Paradox : Clinical Significance of Coexpression of E-cadherin and Vimentin With Regard to Invasion and Metastasis of Breast Cancer. / Yamashita, Nami; Tokunaga, Eriko; Iimori, Makoto; Inoue, Yuka; Tanaka, Kimihiro; Kitao, Hiroyuki; Saeki, Hiroshi; Oki, Eiji; Maehara, Yoshihiko.

In: Clinical Breast Cancer, Vol. 18, No. 5, 01.10.2018, p. e1003-e1009.

Research output: Contribution to journalArticle

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abstract = "E-cadherin and vimentin are regarded as major conventional canonical markers of epithelial–mesenchymal transition. Both E-cadherin– and vimentin-positive tumors had the worst prognosis among all cases. Further, E-cadherin and vimentin protein is colocalized within the same tumor cells, suggesting the existence of an aggressive subpopulation in the primary tumor nest of breast cancer. Background: E-cadherin and vimentin are regarded as major conventional canonical markers of the epithelial–mesenchymal transition. It is commonly assumed that E-cadherin is uniformly lost during the process of epithelial–mesenchymal transition. Breast tumor cells typically invade as a cohesive multicellular unit in a process called collective invasion. The aim of this study was to reveal the clinical importance of the expression pattern of E-cadherin and vimentin in breast cancer. Methods: E-cadherin and vimentin protein expression was evaluated by immunohistochemistry in 176 invasive breast cancer samples. Among these, E-cadherin and vimentin expression were evaluated in the set of primary site and metastatic lymph nodes in 65 cases. In addition, E-cadherin and vimentin expression were analyzed by confocal laser scanning microscopy to see E-cadherin and vimentin localization in the breast cancer cells. Results: Both at the primary site and metastatic lymph nodes, both E-cadherin– and vimentin-positive tumors had the worst disease-free and overall survival among all cases. In addition, E-cadherin and vimentin protein is colocalized within the same tumor cells in a human breast cancer specimen. Conclusion: Our present data suggest the existence of an aggressive subpopulation in the primary tumor nest of breast cancer.",
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AU - Iimori, Makoto

AU - Inoue, Yuka

AU - Tanaka, Kimihiro

AU - Kitao, Hiroyuki

AU - Saeki, Hiroshi

AU - Oki, Eiji

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