Epstein-Barr virus (EBV) load and cytokine gene expression in activated T cells of chronic active EBV infection

Shouichi Ohga, Akihiko Nomura, Hidetoshi Takada, Kenji Ihara, Kiyoshi Kawakami, Fumio Yanai, Yasushi Takahata, Tamami Tanaka, Naoki Kasuga, Toshiro Hara

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Abstract

To identify the role of T cells in chronic active Epstein-Barr virus (EBV) infection, EBV and cytokine gene expression was quantified by use of real-time polymerase chain reaction (PCR) among 6 patients who fulfilled the diagnostic criteria for chronic active EBV infection. Four of these patients showed clonal expansion of EBV-infected T cells. Quantitative PCR for EBV DNA in peripheral blood of patients with symptomatic chronic active EBV infection showed higher copy numbers of virus (mean, 1.45 × 105 copies/mL) than were seen in blood from patients with infectious mononucleosis (3.08 × 103 copies/mL) or with EBV-associated hemophagocytosis (2.95 × 104 copies/mL). Fractionated CD3+ HLA-DR+ cells from patients with chronic active EBV infection contained higher copy numbers than did CD3+ HLA-DR- cells. Quantitative PCR for cytokines revealed that interferon-γ, interleukin (IL)-2, IL-10, and transforming growth factor-β genes were expressed at higher levels in HLA-DR+ than in HLA-DR- T cells. These results suggest that activated T cells in chronic active EBV infection expressed high levels of EBV DNA and both Th1 and Th2 cytokines. EBV-infected T cells may contribute to the unbalanced cytokine profiles of chronic mononucleosis.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Infectious Diseases
Volume183
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

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Epstein-Barr Virus Infections
Human Herpesvirus 4
HLA-DR Antigens
Cytokines
T-Lymphocytes
Gene Expression
Polymerase Chain Reaction
Infectious Mononucleosis
DNA
Transforming Growth Factors
Interleukin-10
Interferons
Interleukin-2
Real-Time Polymerase Chain Reaction
Viruses
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases

Cite this

Epstein-Barr virus (EBV) load and cytokine gene expression in activated T cells of chronic active EBV infection. / Ohga, Shouichi; Nomura, Akihiko; Takada, Hidetoshi; Ihara, Kenji; Kawakami, Kiyoshi; Yanai, Fumio; Takahata, Yasushi; Tanaka, Tamami; Kasuga, Naoki; Hara, Toshiro.

In: Journal of Infectious Diseases, Vol. 183, No. 1, 01.01.2001, p. 1-7.

Research output: Contribution to journalArticle

Ohga, S, Nomura, A, Takada, H, Ihara, K, Kawakami, K, Yanai, F, Takahata, Y, Tanaka, T, Kasuga, N & Hara, T 2001, 'Epstein-Barr virus (EBV) load and cytokine gene expression in activated T cells of chronic active EBV infection', Journal of Infectious Diseases, vol. 183, no. 1, pp. 1-7. https://doi.org/10.1086/317653
Ohga, Shouichi ; Nomura, Akihiko ; Takada, Hidetoshi ; Ihara, Kenji ; Kawakami, Kiyoshi ; Yanai, Fumio ; Takahata, Yasushi ; Tanaka, Tamami ; Kasuga, Naoki ; Hara, Toshiro. / Epstein-Barr virus (EBV) load and cytokine gene expression in activated T cells of chronic active EBV infection. In: Journal of Infectious Diseases. 2001 ; Vol. 183, No. 1. pp. 1-7.
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