TY - JOUR
T1 - Equarin, a novel soluble molecule expressed with polarity at chick embryonic lens equator, is involved in eye formation
AU - Mu, Hong
AU - Ohta, Kunimasa
AU - Kuriyama, Sei
AU - Shimada, Naoko
AU - Tanihara, Hidenobu
AU - Yasuda, Kunio
AU - Tanaka, Hideaki
N1 - Funding Information:
We thank Dr. H. Kondoh for a chick E7 lens λgt 10 cDNA library, Dr. K.G. Johnson for pCS2+-MT-Ncad vector, and Dr. M. Perron for Xenopus Pax2 cDNA. We also thank Dr. D. Campbell for his critical reading of the manuscript and Dr. T. Okafuji for his help and advice. We also wish to thank the Developmental Studies Hybridoma Bank at the University of Iowa for antibodies. This work was supported in part by PRESTO of the Japan Science and Technology Corporation (grant to K.O.) and Grants-in-Aid from the Ministry of Education, Science, Sports, and Culture of Japan to H.T. and K.O.
PY - 2003/2
Y1 - 2003/2
N2 - The lens plays an important role in eye development. To investigate the molecular mechanisms involved, we used signal sequence trap screens with a chicken lens cDNA library and identified a novel secreted molecule, equarin. Equarin encodes consensus repeat domains conserved in human SRPX and mouse Urb. In the embryonic eye, equarin transcript is detected exclusively in the lens, and persists in the lens equatorial region in a high-dorsal-to-low-ventral gradient. In vitro analysis of equarin protein indicated that after translation, it is modified, cleaved, and secreted to extracellular locations. Microinjection of equarin mRNA into Xenopus embryos induced abnormal eye development. These data suggest that equarin is involved in eye formation.
AB - The lens plays an important role in eye development. To investigate the molecular mechanisms involved, we used signal sequence trap screens with a chicken lens cDNA library and identified a novel secreted molecule, equarin. Equarin encodes consensus repeat domains conserved in human SRPX and mouse Urb. In the embryonic eye, equarin transcript is detected exclusively in the lens, and persists in the lens equatorial region in a high-dorsal-to-low-ventral gradient. In vitro analysis of equarin protein indicated that after translation, it is modified, cleaved, and secreted to extracellular locations. Microinjection of equarin mRNA into Xenopus embryos induced abnormal eye development. These data suggest that equarin is involved in eye formation.
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U2 - 10.1016/S0925-4773(02)00423-9
DO - 10.1016/S0925-4773(02)00423-9
M3 - Article
C2 - 12559487
AN - SCOPUS:0037310411
SN - 0925-4773
VL - 120
SP - 143
EP - 155
JO - Cell Differentiation and Development
JF - Cell Differentiation and Development
IS - 2
ER -