ERRγ tethers strongly bisphenol A and 4-α-cumylphenol in an induced-fit manner

Ayami Matsushima, Takamasa Teramoto, Hiroyuki Okada, Xiaohui Liu, Takatoshi Tokunaga, Yoshimitsu Kakuta, Yasuyuki Shimohigashi

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

A receptor-binding assay and X-ray crystal structure analysis demonstrated that the endocrine disruptor bisphenol A (BPA) strongly binds to human estrogen-related receptor γ (ERRγ). BPA is well anchored to the ligand-binding pocket, forming hydrogen bonds with its two phenol-hydroxyl groups. In this study, we found that 4-α-cumylphenol lacking one of its phenol-hydroxyl groups also binds to ERRγ very strongly. The 2.0 Å crystal structure of the 4-α-cumylphenol/ERRγ complex clearly revealed that ERRγ's Leu345-β-isopropyl plays a role in the tight binding of 4-α-cumylphenol and BPA, rotating in a back-and-forth induced-fit manner.

Original languageEnglish
Pages (from-to)408-413
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume373
Issue number3
DOIs
Publication statusPublished - Aug 29 2008

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Estrogen Receptors
Estrogens
Phenol
Hydroxyl Radical
Crystal structure
Endocrine Disruptors
Hydrogen
Assays
Hydrogen bonds
X-Rays
Ligands
X rays
4-cumylphenol
bisphenol A

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

ERRγ tethers strongly bisphenol A and 4-α-cumylphenol in an induced-fit manner. / Matsushima, Ayami; Teramoto, Takamasa; Okada, Hiroyuki; Liu, Xiaohui; Tokunaga, Takatoshi; Kakuta, Yoshimitsu; Shimohigashi, Yasuyuki.

In: Biochemical and Biophysical Research Communications, Vol. 373, No. 3, 29.08.2008, p. 408-413.

Research output: Contribution to journalArticle

Matsushima, Ayami ; Teramoto, Takamasa ; Okada, Hiroyuki ; Liu, Xiaohui ; Tokunaga, Takatoshi ; Kakuta, Yoshimitsu ; Shimohigashi, Yasuyuki. / ERRγ tethers strongly bisphenol A and 4-α-cumylphenol in an induced-fit manner. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 373, No. 3. pp. 408-413.
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AU - Kakuta, Yoshimitsu

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