TY - JOUR
T1 - Erratum
T2 - PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions (J. Biol. Chem. (2020) 295 (12247-12261) DOI: 10.1074/jbc.RA119.011870)
AU - Ishii, Takashi
AU - Igawa, Tatsuhiro
AU - Hayakawa, Hiroshi
AU - Fujita, Tsugumi
AU - Sekiguchi, Mutsuo
AU - Nakabeppu, Yusaku
N1 - Publisher Copyright:
© 2021 American Society for Biochemistry and Molecular Biology Inc.. All rights reserved.
PY - 2021/12/1
Y1 - 2021/12/1
N2 - The following legend has been corrected. In the legend corresponding to Figure 8B, where it reads: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus all others, ###p < 0.0001 versus WT. It should read: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus PCBP2-KO + WT**p < 0.001 versus PCBP2- KO + WT, ###p < 0.0001 versus WT.
AB - The following legend has been corrected. In the legend corresponding to Figure 8B, where it reads: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus all others, ###p < 0.0001 versus WT. It should read: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus PCBP2-KO + WT**p < 0.001 versus PCBP2- KO + WT, ###p < 0.0001 versus WT.
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U2 - 10.1016/j.jbc.2021.101447
DO - 10.1016/j.jbc.2021.101447
M3 - Comment/debate
C2 - 34839120
AN - SCOPUS:85121275682
VL - 297
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 6
M1 - 101447
ER -