Erratum: PCBP1 and PCBP2 both bind heavily oxidized RNA but cause opposing outcomes, suppressing or increasing apoptosis under oxidative conditions (J. Biol. Chem. (2020) 295 (12247-12261) DOI: 10.1074/jbc.RA119.011870)

Takashi Ishii, Tatsuhiro Igawa, Hiroshi Hayakawa, Tsugumi Fujita, Mutsuo Sekiguchi, Yusaku Nakabeppu

Research output: Contribution to journalComment/debatepeer-review

Abstract

The following legend has been corrected. In the legend corresponding to Figure 8B, where it reads: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus all others, ###p < 0.0001 versus WT. It should read: B, cleavage of PARP-1 after H2O2 exposure. Cells (WT, PCBP2-KO, PCBP2-KO + WT and PCBP2-KO + KH1DD) were incubated with 0.2 mM H2O2. At the indicated times, the cells were recovered and boiled in SDS lysis buffer. The samples were subjected to SDS-PAGE followed by Western blotting using an anti-PARP-1 antibody (top). The band intensities shown in the blots were measured by Image Quant TL, and the percentage of cleaved PARP1 was determined (bottom). Data from three independent experiments are shown as the mean ± SE. A two-way ANOVA was performed as a statistical analysis.***p < 0.0001 versus PCBP2-KO + WT**p < 0.001 versus PCBP2- KO + WT, ###p < 0.0001 versus WT.

Original languageEnglish
Article number101447
JournalJournal of Biological Chemistry
Volume297
Issue number6
DOIs
Publication statusPublished - Dec 1 2021

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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