Error-prone bypass of certain DNA lesions by the human DNA polymerase κ

Eiji Ohashi, Tomoo Ogi, Rika Kusumoto, Shigenori Iwai, Chikahide Masutani, Fumio Hanaoka, Haruo Ohmori

Research output: Contribution to journalArticle

247 Citations (Scopus)

Abstract

The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.

Original languageEnglish
Pages (from-to)1589-1594
Number of pages6
JournalGenes and Development
Volume14
Issue number13
Publication statusPublished - Jul 1 2000
Externally publishedYes

Fingerprint

DNA-Directed DNA Polymerase
Pyrimidine Dimers
2-Acetylaminofluorene
Thymine
Escherichia coli Proteins
DNA
Cisplatin
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Ohashi, E., Ogi, T., Kusumoto, R., Iwai, S., Masutani, C., Hanaoka, F., & Ohmori, H. (2000). Error-prone bypass of certain DNA lesions by the human DNA polymerase κ. Genes and Development, 14(13), 1589-1594.

Error-prone bypass of certain DNA lesions by the human DNA polymerase κ. / Ohashi, Eiji; Ogi, Tomoo; Kusumoto, Rika; Iwai, Shigenori; Masutani, Chikahide; Hanaoka, Fumio; Ohmori, Haruo.

In: Genes and Development, Vol. 14, No. 13, 01.07.2000, p. 1589-1594.

Research output: Contribution to journalArticle

Ohashi, E, Ogi, T, Kusumoto, R, Iwai, S, Masutani, C, Hanaoka, F & Ohmori, H 2000, 'Error-prone bypass of certain DNA lesions by the human DNA polymerase κ', Genes and Development, vol. 14, no. 13, pp. 1589-1594.
Ohashi E, Ogi T, Kusumoto R, Iwai S, Masutani C, Hanaoka F et al. Error-prone bypass of certain DNA lesions by the human DNA polymerase κ. Genes and Development. 2000 Jul 1;14(13):1589-1594.
Ohashi, Eiji ; Ogi, Tomoo ; Kusumoto, Rika ; Iwai, Shigenori ; Masutani, Chikahide ; Hanaoka, Fumio ; Ohmori, Haruo. / Error-prone bypass of certain DNA lesions by the human DNA polymerase κ. In: Genes and Development. 2000 ; Vol. 14, No. 13. pp. 1589-1594.
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