Error-prone bypass of certain DNA lesions by the human DNA polymerase κ

Eiji Ohashi, Tomoo Ogi, Rika Kusumoto, Shigenori Iwai, Chikahide Masutani, Fumio Hanaoka, Haruo Ohmori

Research output: Contribution to journalArticlepeer-review

255 Citations (Scopus)

Abstract

The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.

Original languageEnglish
Pages (from-to)1589-1594
Number of pages6
JournalGenes and Development
Volume14
Issue number13
Publication statusPublished - Jul 1 2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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