Abstract
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.
Original language | English |
---|---|
Pages (from-to) | 1589-1594 |
Number of pages | 6 |
Journal | Genes and Development |
Volume | 14 |
Issue number | 13 |
Publication status | Published - Jul 1 2000 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology