Error-prone bypass of certain DNA lesions by the human DNA polymerase κ

Eiji Ohashi; Tomoo Ogi; Rika Kusumoto; Shigenori Iwai; Chikahide Masutani; Fumio Hanaoka; Haruo Ohmori

Error-prone bypass of certain DNA lesions by the human DNA polymerase κ

Eiji Ohashi, Tomoo Ogi, Rika Kusumoto, Shigenori Iwai, Chikahide Masutani, Fumio Hanaoka, Haruo Ohmori

Research output: Contribution to journal › Article › peer-review

Abstract

The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.

Original language	English
Pages (from-to)	1589-1594
Number of pages	6
Journal	Genes and Development
Volume	14
Issue number	13
Publication status	Published - Jul 1 2000
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Developmental Biology

Access to Document

Fingerprint Dive into the research topics of 'Error-prone bypass of certain DNA lesions by the human DNA polymerase κ'. Together they form a unique fingerprint.

Cite this

@article{22626dd2985f45f5b579fe6c6df7e595,

title = "Error-prone bypass of certain DNA lesions by the human DNA polymerase κ",

abstract = "The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.",

author = "Eiji Ohashi and Tomoo Ogi and Rika Kusumoto and Shigenori Iwai and Chikahide Masutani and Fumio Hanaoka and Haruo Ohmori",

year = "2000",

month = jul,

day = "1",

language = "English",

volume = "14",

pages = "1589--1594",

journal = "Genes and Development",

issn = "0890-9369",

publisher = "Cold Spring Harbor Laboratory Press",

number = "13",

}

TY - JOUR

T1 - Error-prone bypass of certain DNA lesions by the human DNA polymerase κ

AU - Ohashi, Eiji

AU - Ogi, Tomoo

AU - Kusumoto, Rika

AU - Iwai, Shigenori

AU - Masutani, Chikahide

AU - Hanaoka, Fumio

AU - Ohmori, Haruo

PY - 2000/7/1

Y1 - 2000/7/1

N2 - The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.

AB - The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.

UR - http://www.scopus.com/inward/record.url?scp=0034235584&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034235584&partnerID=8YFLogxK

M3 - Article

C2 - 10887153

AN - SCOPUS:0034235584

VL - 14

SP - 1589

EP - 1594

JO - Genes and Development

JF - Genes and Development

SN - 0890-9369

IS - 13

ER -