Abstract
The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.
| Original language | English |
|---|---|
| Pages (from-to) | 1589-1594 |
| Number of pages | 6 |
| Journal | Genes and Development |
| Volume | 14 |
| Issue number | 13 |
| Publication status | Published - Jul 1 2000 |
| Externally published | Yes |
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All Science Journal Classification (ASJC) codes
- Genetics
- Developmental Biology
Cite this
Error-prone bypass of certain DNA lesions by the human DNA polymerase κ. / Ohashi, Eiji; Ogi, Tomoo; Kusumoto, Rika; Iwai, Shigenori; Masutani, Chikahide; Hanaoka, Fumio; Ohmori, Haruo.
In: Genes and Development, Vol. 14, No. 13, 01.07.2000, p. 1589-1594.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Error-prone bypass of certain DNA lesions by the human DNA polymerase κ
AU - Ohashi, Eiji
AU - Ogi, Tomoo
AU - Kusumoto, Rika
AU - Iwai, Shigenori
AU - Masutani, Chikahide
AU - Hanaoka, Fumio
AU - Ohmori, Haruo
PY - 2000/7/1
Y1 - 2000/7/1
N2 - The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.
AB - The Escherichia coli protein DinB is a newly identified error-prone DNA polymerase. Recently, a human homolog of DinB was identified and named DINB1. We report that the DINB1 gene encodes a DNA polymerase (designated polK), which incorporates mismatched bases on a nondamaged template with a high frequency. Moreover, polk bypasses an abasic site and N-2-acetylaminofluorene (AAF)-adduct in an error-prone manner but does not bypass a cis-syn or (6-4) thymine-thymine dimer or a cisplatin-adduct. Therefore, our results implicate an important role for polk in the mutagenic bypass of certain types of DNA lesions.
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M3 - Article
VL - 14
SP - 1589
EP - 1594
JO - Genes and Development
JF - Genes and Development
SN - 0890-9369
IS - 13
ER -