Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage

Daisuke Sugiyama, Minetaro Ogawa, Imiko Hirose, Thierry Jaffredo, Ken ichi Arai, Kohichiro Tsuji

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Erythropoiesis is characterized by 2 waves of production during mouse embryogenesis: a primitive one originating from the yolk sac (YS) and a definitive one produced from both the YS and the embryo proper. How the latter wave is generated remains unclear. To investigate our hypothesis that endothelial cells (ECs) could generate erythroid cells, we designed a method to label ECs at 10 days after coitus. This labeling method associates 2 techniques: an intracardiac inoculation that allows molecules to be delivered into the bloodstream followed by a whole-embryo culture period. Dil-conjugated acetylated low-density lipoproteins (Ac-LDL-Dil) were used to specifically tag ECs from the inside. One hour after inoculation, Dil staining was found along the entire endothelial tree. Fluorescence-activated cell sorter (FACS) analysis revealed that Dil+ cells were CD31+, CD34+, and CD45-, an antigen makeup characteristic of the endothelial lineage. Twelve hours after inoculation, 43% of Dil+ circulating cells belonged to the erythroid lineage. These cells expressed Ter119 and displayed an adult globin chain arrangement; thus they belonged to the definitive lineage as confirmed in erythroid colony formation. The remaining cells likely represent committed white blood cells or multipotent progenitors, as revealed by a mixed-colony formation. Beyond the 29-somite stage, the proportion of Dil+ erythroid cells gradually decreased. These results demonstrate the generation of hematopoietic cells from an endothelial intermediate, using in vivo tracing. We provide evidence for a release of these cells into the circulation and hypothesize that these cells are able to colonize the fetal liver and generate definitive erythrocytes in vivo.

Original languageEnglish
Pages (from-to)4733-4738
Number of pages6
JournalBlood
Volume101
Issue number12
Publication statusPublished - Jun 15 2003
Externally publishedYes

Fingerprint

Erythropoiesis
Endothelial cells
Endothelial Cells
CD34 Antigens
CD45 Antigens
Globins
Yolk Sac
Erythroid Cells
Liver
Labeling
Labels
Blood
Fluorescence
Cells
Embryonic Structures
Molecules
Somites
Coitus
acetyl-LDL
Embryonic Development

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Sugiyama, D., Ogawa, M., Hirose, I., Jaffredo, T., Arai, K. I., & Tsuji, K. (2003). Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage. Blood, 101(12), 4733-4738.

Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage. / Sugiyama, Daisuke; Ogawa, Minetaro; Hirose, Imiko; Jaffredo, Thierry; Arai, Ken ichi; Tsuji, Kohichiro.

In: Blood, Vol. 101, No. 12, 15.06.2003, p. 4733-4738.

Research output: Contribution to journalArticle

Sugiyama, D, Ogawa, M, Hirose, I, Jaffredo, T, Arai, KI & Tsuji, K 2003, 'Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage', Blood, vol. 101, no. 12, pp. 4733-4738.
Sugiyama D, Ogawa M, Hirose I, Jaffredo T, Arai KI, Tsuji K. Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage. Blood. 2003 Jun 15;101(12):4733-4738.
Sugiyama, Daisuke ; Ogawa, Minetaro ; Hirose, Imiko ; Jaffredo, Thierry ; Arai, Ken ichi ; Tsuji, Kohichiro. / Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage. In: Blood. 2003 ; Vol. 101, No. 12. pp. 4733-4738.
@article{e9dfb18fe5fc446d8e1ef7d042882527,
title = "Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage",
abstract = "Erythropoiesis is characterized by 2 waves of production during mouse embryogenesis: a primitive one originating from the yolk sac (YS) and a definitive one produced from both the YS and the embryo proper. How the latter wave is generated remains unclear. To investigate our hypothesis that endothelial cells (ECs) could generate erythroid cells, we designed a method to label ECs at 10 days after coitus. This labeling method associates 2 techniques: an intracardiac inoculation that allows molecules to be delivered into the bloodstream followed by a whole-embryo culture period. Dil-conjugated acetylated low-density lipoproteins (Ac-LDL-Dil) were used to specifically tag ECs from the inside. One hour after inoculation, Dil staining was found along the entire endothelial tree. Fluorescence-activated cell sorter (FACS) analysis revealed that Dil+ cells were CD31+, CD34+, and CD45-, an antigen makeup characteristic of the endothelial lineage. Twelve hours after inoculation, 43{\%} of Dil+ circulating cells belonged to the erythroid lineage. These cells expressed Ter119 and displayed an adult globin chain arrangement; thus they belonged to the definitive lineage as confirmed in erythroid colony formation. The remaining cells likely represent committed white blood cells or multipotent progenitors, as revealed by a mixed-colony formation. Beyond the 29-somite stage, the proportion of Dil+ erythroid cells gradually decreased. These results demonstrate the generation of hematopoietic cells from an endothelial intermediate, using in vivo tracing. We provide evidence for a release of these cells into the circulation and hypothesize that these cells are able to colonize the fetal liver and generate definitive erythrocytes in vivo.",
author = "Daisuke Sugiyama and Minetaro Ogawa and Imiko Hirose and Thierry Jaffredo and Arai, {Ken ichi} and Kohichiro Tsuji",
year = "2003",
month = "6",
day = "15",
language = "English",
volume = "101",
pages = "4733--4738",
journal = "Blood",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "12",

}

TY - JOUR

T1 - Erythropoiesis from acetyl LDL incorporating endothelial cells at the preliver stage

AU - Sugiyama, Daisuke

AU - Ogawa, Minetaro

AU - Hirose, Imiko

AU - Jaffredo, Thierry

AU - Arai, Ken ichi

AU - Tsuji, Kohichiro

PY - 2003/6/15

Y1 - 2003/6/15

N2 - Erythropoiesis is characterized by 2 waves of production during mouse embryogenesis: a primitive one originating from the yolk sac (YS) and a definitive one produced from both the YS and the embryo proper. How the latter wave is generated remains unclear. To investigate our hypothesis that endothelial cells (ECs) could generate erythroid cells, we designed a method to label ECs at 10 days after coitus. This labeling method associates 2 techniques: an intracardiac inoculation that allows molecules to be delivered into the bloodstream followed by a whole-embryo culture period. Dil-conjugated acetylated low-density lipoproteins (Ac-LDL-Dil) were used to specifically tag ECs from the inside. One hour after inoculation, Dil staining was found along the entire endothelial tree. Fluorescence-activated cell sorter (FACS) analysis revealed that Dil+ cells were CD31+, CD34+, and CD45-, an antigen makeup characteristic of the endothelial lineage. Twelve hours after inoculation, 43% of Dil+ circulating cells belonged to the erythroid lineage. These cells expressed Ter119 and displayed an adult globin chain arrangement; thus they belonged to the definitive lineage as confirmed in erythroid colony formation. The remaining cells likely represent committed white blood cells or multipotent progenitors, as revealed by a mixed-colony formation. Beyond the 29-somite stage, the proportion of Dil+ erythroid cells gradually decreased. These results demonstrate the generation of hematopoietic cells from an endothelial intermediate, using in vivo tracing. We provide evidence for a release of these cells into the circulation and hypothesize that these cells are able to colonize the fetal liver and generate definitive erythrocytes in vivo.

AB - Erythropoiesis is characterized by 2 waves of production during mouse embryogenesis: a primitive one originating from the yolk sac (YS) and a definitive one produced from both the YS and the embryo proper. How the latter wave is generated remains unclear. To investigate our hypothesis that endothelial cells (ECs) could generate erythroid cells, we designed a method to label ECs at 10 days after coitus. This labeling method associates 2 techniques: an intracardiac inoculation that allows molecules to be delivered into the bloodstream followed by a whole-embryo culture period. Dil-conjugated acetylated low-density lipoproteins (Ac-LDL-Dil) were used to specifically tag ECs from the inside. One hour after inoculation, Dil staining was found along the entire endothelial tree. Fluorescence-activated cell sorter (FACS) analysis revealed that Dil+ cells were CD31+, CD34+, and CD45-, an antigen makeup characteristic of the endothelial lineage. Twelve hours after inoculation, 43% of Dil+ circulating cells belonged to the erythroid lineage. These cells expressed Ter119 and displayed an adult globin chain arrangement; thus they belonged to the definitive lineage as confirmed in erythroid colony formation. The remaining cells likely represent committed white blood cells or multipotent progenitors, as revealed by a mixed-colony formation. Beyond the 29-somite stage, the proportion of Dil+ erythroid cells gradually decreased. These results demonstrate the generation of hematopoietic cells from an endothelial intermediate, using in vivo tracing. We provide evidence for a release of these cells into the circulation and hypothesize that these cells are able to colonize the fetal liver and generate definitive erythrocytes in vivo.

UR - http://www.scopus.com/inward/record.url?scp=0038454601&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0038454601&partnerID=8YFLogxK

M3 - Article

C2 - 12595314

AN - SCOPUS:0038454601

VL - 101

SP - 4733

EP - 4738

JO - Blood

JF - Blood

SN - 0006-4971

IS - 12

ER -