Erythropoietin attenuated high glucose-induced apoptosis in cultured human aortic endothelial cells

Naotaka Sekiguchi, Toyoshi Inoguchi, Kunihisa Kobayashi, Noriyuki Sonoda, Hajime Nawata

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

High glucose-induced apoptosis in vascular endothelial cells may contribute to the acceleration of atherosclerosis associated with diabetes. Here, we show that erythropoietin attenuates high glucose-induced apoptosis in cultured human aortic endothelial cells (HAECs). Exposure of HAECs to high glucose level for 72 h significantly increased the number of apoptotic cells compared with normal glucose level, as evaluated by TUNEL assay. Simultaneous addition of erythropoietin (100 U/ml) significantly attenuated high glucose-induced apoptosis. In parallel, exposure to high glucose level induced caspase-3 activation and erythropoietin also prevented it. Erythropoietin stimulated Akt phosphorylation in a dose-dependent manner (1-100 U/ml). PI3 kinase inhibitor, wortmannin or LY294002 eliminated erythropoietin's inhibitory effect on caspase-3 activity. In conclusion, erythropoietin may attenuate high glucose-induced endothelial cell apoptosis via PI-3 kinase pathway. Replacing therapy with erythropoietin is often used for correction of renal anemia, but may have potential in preventing atherosclerosis in diabetic patients with end-stage renal failure.

Original languageEnglish
Pages (from-to)218-222
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume334
Issue number1
DOIs
Publication statusPublished - Aug 19 2005

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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