Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis

Yasunori Masuike, Koji Tanaka, Tomoki Makino, Makoto Yamasaki, Yasuhiro Miyazaki, Tsuyoshi Takahashi, Yukinori Kurokawa, Kiyokazu Nakajima, Masaki Mori, Yuichiro Doki

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Abstract

Alterations in mitochondrial DNA (mtDNA) copy numbers in various human cancers have been studied, but any such changes in esophageal squamous cell carcinoma (ESCC) are not established. In the present study, we investigated the correlation of mtDNA copy number with clinicopathologic features, prognosis, and malignant potential of ESCC. MtDNA copy numbers of resected specimens from 80 patients treated with radical esophagectomy were measured by quantitative real-time PCR analyses. Human ESCC cells, TE8 and TE11, were cultured, and depletion of mtDNA content was induced by knockdown of mitochondrial transcription factor A expression or treatment with ethidium bromide. The mRNA and protein expression, proliferation, invasion, and cell cycle were investigated. The results showed that the mtDNA copy number of cancerous portions was 56.0 (37.4–234.5) percent that of noncancerous parts and significantly lower (p<0.01). Low mtDNA copy number in resected cancerous tissues was significantly correlated with pathological depth of tumor invasion (p = 0.045) and pathological stage (p = 0.025). Patients with lower mtDNA copy number had significantly poorer 5-year overall survival compared to patients with higher levels (p<0.01). The mtDNA-depleted TE8 and TE11 cells had morphological changes and proliferated more slowly than control cells under normoxia but proliferated at almost the same rate under hypoxic conditions. In mtDNA-depleted cells, E-cadherin mRNA expression was decreased, and N-cadherin, vimentin, zeb-1, and cd44 mRNA expression was increased. Immunoblotting and flow cytometry analysis also showed downregulated E-cadherin and upregulated N-cadherin and CD44 protein in mtDNA-depleted cells. Moreover, mtDNA-depleted cells had enhanced invasion, migration, and sphere formation abilities, and the cell cycle arrest at G0/G1 phase was induced in these cells. These results suggested that mtDNA-depleted ESCC cells had mesenchymal characteristics, cancer stemness, and tolerance to hypoxia, which played important role in cancer progression. In conclusion, a low copy number of mtDNA is associated with tumor progression in ESCC.

Original languageEnglish
Article numbere0193159
JournalPloS one
Volume13
Issue number2
DOIs
Publication statusPublished - Feb 1 2018
Externally publishedYes

Fingerprint

squamous cell carcinoma
Mitochondrial DNA
prognosis
mitochondrial DNA
stems
cadherins
Cadherins
neoplasms
cells
Neoplasms
Messenger RNA
Esophageal Squamous Cell Carcinoma
Epithelial Cells
Tumors
Cells
Cell Cycle Resting Phase
Esophagectomy
normoxia
Ethidium
Flow cytometry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis. / Masuike, Yasunori; Tanaka, Koji; Makino, Tomoki; Yamasaki, Makoto; Miyazaki, Yasuhiro; Takahashi, Tsuyoshi; Kurokawa, Yukinori; Nakajima, Kiyokazu; Mori, Masaki; Doki, Yuichiro.

In: PloS one, Vol. 13, No. 2, e0193159, 01.02.2018.

Research output: Contribution to journalArticle

Masuike, Y, Tanaka, K, Makino, T, Yamasaki, M, Miyazaki, Y, Takahashi, T, Kurokawa, Y, Nakajima, K, Mori, M & Doki, Y 2018, 'Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis', PloS one, vol. 13, no. 2, e0193159. https://doi.org/10.1371/journal.pone.0193159
Masuike, Yasunori ; Tanaka, Koji ; Makino, Tomoki ; Yamasaki, Makoto ; Miyazaki, Yasuhiro ; Takahashi, Tsuyoshi ; Kurokawa, Yukinori ; Nakajima, Kiyokazu ; Mori, Masaki ; Doki, Yuichiro. / Esophageal squamous cell carcinoma with low mitochondrial copy number has mesenchymal and stem-like characteristics, and contributes to poor prognosis. In: PloS one. 2018 ; Vol. 13, No. 2.
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AU - Miyazaki, Yasuhiro

AU - Takahashi, Tsuyoshi

AU - Kurokawa, Yukinori

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AU - Mori, Masaki

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