Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

Takuya Imamura; Thi My Anh Neildez; Catherine Thenevin; Andras Paldi

doi:10.1186/1471-2199-5-4

Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

Takuya Imamura, Thi My Anh Neildez, Catherine Thenevin, Andras Paldi

Stem Cell Biology and Medicine

Research output: Contribution to journal › Article › peer-review

20 Citations (Scopus)

Abstract

Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.

Original language	English
Article number	4
Journal	BMC Molecular Biology
Volume	5
DOIs	https://doi.org/10.1186/1471-2199-5-4
Publication status	Published - Jun 16 2004

All Science Journal Classification (ASJC) codes

Molecular Biology

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title = "Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development",

abstract = "Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.",

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T1 - Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

AU - Imamura, Takuya

AU - Neildez, Thi My Anh

AU - Thenevin, Catherine

AU - Paldi, Andras

PY - 2004/6/16

Y1 - 2004/6/16

N2 - Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.

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