Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development

Takuya Imamura, Thi My Anh Neildez, Catherine Thenevin, Andras Paldi

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18 Citations (Scopus)

Abstract

Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.

Original languageEnglish
Article number4
JournalBMC Molecular Biology
Volume5
DOIs
Publication statusPublished - Jun 16 2004

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All Science Journal Classification (ASJC) codes

  • Molecular Biology

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