TY - JOUR
T1 - Essential role for poly (ADP-ribosyl)ation in mouse preimplantation development
AU - Imamura, Takuya
AU - Neildez, Thi My Anh
AU - Thenevin, Catherine
AU - Paldi, Andras
PY - 2004/6/16
Y1 - 2004/6/16
N2 - Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.
AB - Background: Poly (ADP-ribosyl)ation is a covalent modification of many nuclear proteins. It has a strong chromatin modifying potential involved in DNA repair, transcription and replication. Its role during preimplantation development is unknown. Results: We have observed strong but transient synthesis of poly ADP-ribose polymers on decondensing chromosomes of fertilized and parthenogenetically activated mouse oocytes. Inhibition of this transient upregulation with a specific enzyme inhibitor, 3-aminobenzamide, has long-term effects on the postimplantation development of the embryos. In addition, inhibition of poly (ADP-ribosyl)ation at the 4-8 cell stage selectively blocks morula compaction. Conclusion: These observations suggest that poly (ADP-ribosyl)ation is involved in the epigenetic chromatin remodeling in the zygote.
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U2 - 10.1186/1471-2199-5-4
DO - 10.1186/1471-2199-5-4
M3 - Article
C2 - 15200685
AN - SCOPUS:26444509875
VL - 5
JO - BMC Molecular Biology
JF - BMC Molecular Biology
SN - 1471-2199
M1 - 4
ER -