Essential role of Toll-like receptors for dendritic cell and NK1.1+ cell-dependent activation of type 1 immunity by Lactobacillus pentosus strain S-PT84

Shin ichi Koizumi, Daiko Wakita, Tomomi Sato, Rieko Mitamura, Takayuki Izumo, Hiroshi Shibata, Yoshinobu Kiso, Kenji Chamoto, Yuji Togashi, Hidemitsu Kitamura, Takashi Nishimura

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Activation of type 1 immunity plays a critical role in host defense mechanisms against infectious disease and tumor. Lactic acid bacteria, existing in the gastrointestinal tract, are one of the powerful tools to induce a type-1-dominant immunity, which may improve Th2-dependent allergic diseases. In the present work, we found that an oral intake of Lactobacillus pentosus strain, S-PT84 into mice significantly enhanced NK activity of spleen cells in vivo. We further revealed that NK1.1 positive NK cells and NKT cells are responsible cells for producing IFN-γ after stimulation with S-PT84 in vitro. S-PT84 induced IFN-γ-producing cells through activation of IL-12 production by CD11c+DCs in Toll-like receptor (TLR) 2- and/or TLR4-dependent manner. Interestingly, direct interaction between DCs and NK1.1+ cells was also essential for the IFN-γ production in response to the S-PT84 stimulation. Therefore, we concluded that S-PT84 effectively promoted type 1 immunity through IL-12 and IFN-γ which were produced by DCs and NK1.1+ cells, respectively. Thus, S-PT84 would be a nice immune modulator for improving immunobalance, which plays a pivotal role for controlling allergy, infectious diseases and tumor.

Original languageEnglish
Pages (from-to)14-19
Number of pages6
JournalImmunology Letters
Volume120
Issue number1-2
DOIs
Publication statusPublished - Sep 30 2008
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

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