Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation

Satoshi Fukumoto, Aya Yamada, Kazuaki Nonaka, Yoshihiko Yamada

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

During tooth development, dental epithelial cells interact with extracellular matrix components, such as the basement membrane and enamel matrix. Ameloblastin, an enamel matrix protein, plays a crucial role in maintaining the ameloblast differentiation state and is essential for enamel formation. Ameloblastin-null mice developed severe enamel hypoplasia. In mutant mice, dental epithelial cells started to differentiate into ameloblasts, but ameloblasts soon lost cell polarity, proliferated, and formed multiple cell layers, indicative of some aspects of preameloblast phenotypes. In addition, the expression of amelogenin, another component of the enamel matrix, was specifically reduced in mutant ameloblasts. More than 20% of amelobastin-null mice developed odontogenic tumors. We also found that recombinant ameloblastin specifically bound to ameloblasts and inhibited proliferation of dental epithelial cells. These results suggest that ameloblastin is an important regulator to maintain the differentiation state of ameloblasts.

Original languageEnglish
Pages (from-to)189-195
Number of pages7
JournalCells Tissues Organs
Volume181
Issue number3-4
DOIs
Publication statusPublished - Apr 1 2006

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Ameloblasts
Dental Enamel
Tooth
Epithelial Cells
Dental Enamel Hypoplasia
Amelogenin
Odontogenic Tumors
Cell Polarity
Basement Membrane
Extracellular Matrix
Phenotype

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Histology

Cite this

Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation. / Fukumoto, Satoshi; Yamada, Aya; Nonaka, Kazuaki; Yamada, Yoshihiko.

In: Cells Tissues Organs, Vol. 181, No. 3-4, 01.04.2006, p. 189-195.

Research output: Contribution to journalArticle

Fukumoto, Satoshi ; Yamada, Aya ; Nonaka, Kazuaki ; Yamada, Yoshihiko. / Essential roles of ameloblastin in maintaining ameloblast differentiation and enamel formation. In: Cells Tissues Organs. 2006 ; Vol. 181, No. 3-4. pp. 189-195.
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