Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1)

H. Yoshikawa, M. Ohishi, S. Kohriki, M. Yoshiura, Y. Ohsaki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.

Original languageEnglish
Pages (from-to)163-168
Number of pages6
JournalEuropean Journal of Cancer Part B: Oral Oncology
Volume33
Issue number3
DOIs
Publication statusPublished - May 1 1997

Fingerprint

Osteosarcoma
Alkaline Phosphatase
Cell Line
Parathyroid Hormone
Phenotype
Inbred BALB C Mouse
Collagen Type I
Jaw
Osteoblasts
Nude Mice
Culture Media
Neoplasms
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1). / Yoshikawa, H.; Ohishi, M.; Kohriki, S.; Yoshiura, M.; Ohsaki, Y.

In: European Journal of Cancer Part B: Oral Oncology, Vol. 33, No. 3, 01.05.1997, p. 163-168.

Research output: Contribution to journalArticle

Yoshikawa, H. ; Ohishi, M. ; Kohriki, S. ; Yoshiura, M. ; Ohsaki, Y. / Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1). In: European Journal of Cancer Part B: Oral Oncology. 1997 ; Vol. 33, No. 3. pp. 163-168.
@article{1214877548b2455098b9ce9dab3beb0b,
title = "Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1)",
abstract = "A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.",
author = "H. Yoshikawa and M. Ohishi and S. Kohriki and M. Yoshiura and Y. Ohsaki",
year = "1997",
month = "5",
day = "1",
doi = "10.1016/S0964-1955(96)00075-9",
language = "English",
volume = "33",
pages = "163--168",
journal = "European Journal of Cancer Part B: Oral Oncology",
issn = "0964-1955",
publisher = "Pergamon Press Ltd.",
number = "3",

}

TY - JOUR

T1 - Establishment and characterisation of an osteoblastic clonal cell line from human mandibular osteosarcoma (HMOS-1)

AU - Yoshikawa, H.

AU - Ohishi, M.

AU - Kohriki, S.

AU - Yoshiura, M.

AU - Ohsaki, Y.

PY - 1997/5/1

Y1 - 1997/5/1

N2 - A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.

AB - A human mandibular osteosarcoma cell line, HMOS-1, with osteoblastic phenotypes and tumor-genicity was established. The cell line showed high alkaline phosphatase (ALP) activity immediately after seeding, with its peak around the 7th to 10th day of culture (1.44 μmol/min/mg protein). Parathyroid hormone (PTH) enhanced the ALP activity as well as intracellular cAMP production in a concentration-dependent manner. The effects of PTH on both ALP activity and cAMP production were expressed more strongly at the end stage of logarithmic cell growth than at the resting stage. 1,25 (OH)2D3 also stimulated the ALP activity, but its effect was low and was not different in any of the different culture stages. When these cells were transplanted to BALB/C nude mice, similar tumours to the original one, with abundant osteoids were observed. However, the synthesis of type I collagen was not detected in the culture medium. The results indicate that the HMOS-1 cell line expresses an immature pre-osteoblastic phenotype. Because of these characteristics, HMOS-1 cells should be useful, not only in studies on the differentiated phenotypes of human osteoblasts, but also in studies on the diagnosis, treatment and aetiology of human osteosarcoma of the jaw.

UR - http://www.scopus.com/inward/record.url?scp=0031147714&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031147714&partnerID=8YFLogxK

U2 - 10.1016/S0964-1955(96)00075-9

DO - 10.1016/S0964-1955(96)00075-9

M3 - Article

C2 - 9307724

AN - SCOPUS:0031147714

VL - 33

SP - 163

EP - 168

JO - European Journal of Cancer Part B: Oral Oncology

JF - European Journal of Cancer Part B: Oral Oncology

SN - 0964-1955

IS - 3

ER -