Establishment and characterization of a Fas-resistant T cell line

Masanori Higuchi, Takahiko Horiuchi, Takuya Sawabe, Shigeru Yoshizawa, Kohei Nagasawa, Yoshiyuki Niho

Research output: Contribution to journalArticle

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Abstract

Fas is a cell surface receptor that controls a signal transduction pathway leading to apoptosis. We established an antihuman Fas monoclonal antibody (mAb)-resistant variant, kit-225-FR, from the human T cell line, kit-225. Flow cytometric analysis revealed that the expression of Fas molecules on kit-225-FR was preserved. The defect in Fas molecule was not detected either by reverse transcription polymerase chain reaction (PCR) analysis of the Fas transcript or by PCR-single strand conformation polymorphism analysis of the Fas gene in kit-225-FR. Although kit-225-FR was resistant to a high concentration of anti-Fas mAb, apoptosis could be induced, as with the wild type, by exogenous C2-ceramide exposure. MORT1/FADD was expressed at wild-type level in kit-225-FR, as determined by Western blot analysis. It therefore appears that the apoptotic signal transduction in kit-225-FR is defective between FADD and the sphingomyelin-ceramide pathway. By comparing the differences from the wild-type kit-225, kit-225-FR would serve as a useful cell line for analyzing Fas-specific signal transduction pathways in detail.

Original languageEnglish
Pages (from-to)22-30
Number of pages9
JournalActa Haematologica
Volume102
Issue number1
DOIs
Publication statusPublished - Sep 14 1999

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Signal Transduction
anti-Fas monoclonal antibody
T-Lymphocytes
Cell Line
Apoptosis
Polymerase Chain Reaction
Sphingomyelins
Ceramides
Cell Surface Receptors
Reverse Transcription
Western Blotting
Monoclonal Antibodies
Genes
N-acetylsphingosine

All Science Journal Classification (ASJC) codes

  • Hematology

Cite this

Higuchi, M., Horiuchi, T., Sawabe, T., Yoshizawa, S., Nagasawa, K., & Niho, Y. (1999). Establishment and characterization of a Fas-resistant T cell line. Acta Haematologica, 102(1), 22-30. https://doi.org/10.1159/000040963

Establishment and characterization of a Fas-resistant T cell line. / Higuchi, Masanori; Horiuchi, Takahiko; Sawabe, Takuya; Yoshizawa, Shigeru; Nagasawa, Kohei; Niho, Yoshiyuki.

In: Acta Haematologica, Vol. 102, No. 1, 14.09.1999, p. 22-30.

Research output: Contribution to journalArticle

Higuchi, M, Horiuchi, T, Sawabe, T, Yoshizawa, S, Nagasawa, K & Niho, Y 1999, 'Establishment and characterization of a Fas-resistant T cell line', Acta Haematologica, vol. 102, no. 1, pp. 22-30. https://doi.org/10.1159/000040963
Higuchi, Masanori ; Horiuchi, Takahiko ; Sawabe, Takuya ; Yoshizawa, Shigeru ; Nagasawa, Kohei ; Niho, Yoshiyuki. / Establishment and characterization of a Fas-resistant T cell line. In: Acta Haematologica. 1999 ; Vol. 102, No. 1. pp. 22-30.
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