Establishment and characterization of a novel orthotopic mouse model for human uterine sarcoma with different metastatic potentials

Shinya Kawabe, Tetsuya Mizutani, Shin Ishikane, Miguel Ernesto Martinez, Yasushi Kiyono, Koichi Miura, Hiroshi Hosoda, Yoshitaka Imamichi, Kenji Kangawa, Kaoru Miyamoto, Yoshio Yoshida

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Uterine sarcomas are rare and aggressive gynecologic tumors with a poor prognosis because of recurrence and metastasis. However, the mechanisms of uterine sarcoma metastasis are largely unknown. To investigate this mechanism, we developed a novel uterine sarcoma tissue-derived orthotopic and metastatic model in KSN nude mice using a green fluorescent protein stably expressed uterine sarcoma cell line, MES-SA. Histological analysis showed that all orthotopic primary tumors were undifferentiated sarcoma. Primary tumors were characterized by high 18F-fluorodeoxyglucose uptake with a positive correlation to the number of pulmonary metastases. In addition, we generated uterine sarcoma cell sublines with high or low metastatic potentials by serial in vivo selection. Microarray analysis between orthotopic tumors with high and low metastatic potentials revealed differential expression of genes related to cell proliferation and migration (TNNT1, COL1A2, and ZIC1). Our model would be useful to compensate for the limited clinical cases of uterine sarcoma and to investigate the molecular mechanisms of metastatic uterine sarcoma.

Original languageEnglish
Pages (from-to)182-190
Number of pages9
JournalCancer Letters
Volume366
Issue number2
DOIs
Publication statusPublished - Oct 1 2015
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

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