Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1

Fumiko Kito, Rieko Oyama, Rei Noguchi, Emi Hattori, Marimu Sakumoto, Makoto Endo, Eisuke Kobayashi, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

Research output: Contribution to journalArticle

Abstract

Extraskeletal osteosarcoma (ESOS) is a rare mesenchymal malignancy producing osteoid and bone in soft tissue without skeletal attachment. ESOS exhibits chemoresistance and poor prognosis, and is distinct from osseous osteosarcoma. The biological characteristics of ESOS are not fully understood, and patient-derived cell lines of ESOS are not available from public cell banks. Here, we established a novel cell line of ESOS and characterized its genetic and biological characteristics as well as examined its response to anti-cancer reagents. The cell line was established using tumor tissue from a 58-year-old female patient with ESOS, and named as NCC-ESOS1-C1. Phenotypes relevant to malignancy such as proliferation and invasion were examined in vitro, and genetic features were evaluated using the NCC Oncopanel assay. The response to inhibitors was monitored by screening of an anti-cancer reagent library. The cells constantly proliferated, showing spheroid formation and invasion capabilities. The NCC Oncopanel revealed the presence of actionable mutations in PIK3CA. Library screening revealed the presence of anti-cancer reagents with significant anti-proliferative effects on NCC-ESOS1-C1 at a low concentration. In conclusion, we established and characterized a novel ESOS cell line, NCC-ESOS1-C1. This cell line will be a useful resource for basic research and preclinical studies.

Original languageEnglish
JournalHuman Cell
DOIs
Publication statusAccepted/In press - Jan 1 2019

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Osteosarcoma
Cell Line
Neoplasms
Early Detection of Cancer
Phenotype
Bone and Bones
Mutation
Research

All Science Journal Classification (ASJC) codes

  • Cell Biology
  • Cancer Research

Cite this

Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1. / Kito, Fumiko; Oyama, Rieko; Noguchi, Rei; Hattori, Emi; Sakumoto, Marimu; Endo, Makoto; Kobayashi, Eisuke; Yoshida, Akihiko; Kawai, Akira; Kondo, Tadashi.

In: Human Cell, 01.01.2019.

Research output: Contribution to journalArticle

Kito, F, Oyama, R, Noguchi, R, Hattori, E, Sakumoto, M, Endo, M, Kobayashi, E, Yoshida, A, Kawai, A & Kondo, T 2019, 'Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1', Human Cell. https://doi.org/10.1007/s13577-019-00291-z
Kito, Fumiko ; Oyama, Rieko ; Noguchi, Rei ; Hattori, Emi ; Sakumoto, Marimu ; Endo, Makoto ; Kobayashi, Eisuke ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi. / Establishment and characterization of novel patient-derived extraskeletal osteosarcoma cell line NCC-ESOS1-C1. In: Human Cell. 2019.
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AU - Endo, Makoto

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