Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line

Fusako Kito, Rieko Oyama, Marimu Sakumoto, Mami Takahashi, Kumiko Shiozawa, Zhiwei Qiao, Hiromi Sakamoto, Takeshi Hirose, Nokitaka Setsu, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

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5 Citations (Scopus)

Abstract

Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the proteome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.

Original languageEnglish
Pages (from-to)528-536
Number of pages9
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume54
Issue number7
DOIs
Publication statusPublished - Aug 1 2018
Externally publishedYes

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All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

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