Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line

Fusako Kito, Rieko Oyama, Marimu Sakumoto, Mami Takahashi, Kumiko Shiozawa, Zhiwei Qiao, Hiromi Sakamoto, Takeshi Hirose, Nokitaka Setsu, Akihiko Yoshida, Akira Kawai, Tadashi Kondo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the proteome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.

Original languageEnglish
Pages (from-to)528-536
Number of pages9
JournalIn Vitro Cellular and Developmental Biology - Animal
Volume54
Issue number7
DOIs
Publication statusPublished - Aug 1 2018

Fingerprint

Osteosarcoma
Heterografts
Cell Line
Neoplasms
Proteome
Tumor Cell Line
Proteomics
Mass Spectrometry
Histology
Cell Culture Techniques
Transplantation
Bone and Bones
Skin
Growth
Research
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line. / Kito, Fusako; Oyama, Rieko; Sakumoto, Marimu; Takahashi, Mami; Shiozawa, Kumiko; Qiao, Zhiwei; Sakamoto, Hiromi; Hirose, Takeshi; Setsu, Nokitaka; Yoshida, Akihiko; Kawai, Akira; Kondo, Tadashi.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 54, No. 7, 01.08.2018, p. 528-536.

Research output: Contribution to journalArticle

Kito, F, Oyama, R, Sakumoto, M, Takahashi, M, Shiozawa, K, Qiao, Z, Sakamoto, H, Hirose, T, Setsu, N, Yoshida, A, Kawai, A & Kondo, T 2018, 'Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line', In Vitro Cellular and Developmental Biology - Animal, vol. 54, no. 7, pp. 528-536. https://doi.org/10.1007/s11626-018-0274-2
Kito, Fusako ; Oyama, Rieko ; Sakumoto, Marimu ; Takahashi, Mami ; Shiozawa, Kumiko ; Qiao, Zhiwei ; Sakamoto, Hiromi ; Hirose, Takeshi ; Setsu, Nokitaka ; Yoshida, Akihiko ; Kawai, Akira ; Kondo, Tadashi. / Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line. In: In Vitro Cellular and Developmental Biology - Animal. 2018 ; Vol. 54, No. 7. pp. 528-536.
@article{782eda6eb0274c03a45d38b89e461318,
title = "Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line",
abstract = "Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the proteome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.",
author = "Fusako Kito and Rieko Oyama and Marimu Sakumoto and Mami Takahashi and Kumiko Shiozawa and Zhiwei Qiao and Hiromi Sakamoto and Takeshi Hirose and Nokitaka Setsu and Akihiko Yoshida and Akira Kawai and Tadashi Kondo",
year = "2018",
month = "8",
day = "1",
doi = "10.1007/s11626-018-0274-2",
language = "English",
volume = "54",
pages = "528--536",
journal = "In Vitro Cellular and Developmental Biology - Animal",
issn = "1071-2690",
publisher = "Springer New York",
number = "7",

}

TY - JOUR

T1 - Establishment and characterization of novel patient-derived osteosarcoma xenograft and cell line

AU - Kito, Fusako

AU - Oyama, Rieko

AU - Sakumoto, Marimu

AU - Takahashi, Mami

AU - Shiozawa, Kumiko

AU - Qiao, Zhiwei

AU - Sakamoto, Hiromi

AU - Hirose, Takeshi

AU - Setsu, Nokitaka

AU - Yoshida, Akihiko

AU - Kawai, Akira

AU - Kondo, Tadashi

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the proteome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.

AB - Osteosarcoma is an aggressive mesenchymal malignancy of the bone. Patient-derived models are essential tools for elucidating the molecular mechanisms associated with poor prognosis and the development of novel anticancer drugs. This study described the establishment of a patient-derived cancer model of osteosarcoma. Primary osteosarcoma tumor tissues were obtained from an osteosarcoma patient and inoculated in the skin of immunodeficient mice, followed by transplantation to other mice upon growth. Cells were maintained in monolayer cultures, and the capability of spheroid formation was assessed by seeding the cells on culture dishes. The invasion ability of cells was monitored by Matrigel assay, and genomic and proteomic backgrounds were examined by mass spectrometry. A cell line was established from patient-derived tumors and showed similar histology to that of the primary tumor tissue. Additionally, these cells formed spheroids on low-attachment tissue-culture dishes and exhibited invasive capabilities, and we confirmed that the genomic backgrounds were similar between patient-derived xenograft tumors and the cell line. Furthermore, the proteome of the patient-derived tumors and the cells exhibited similar, but not identical, patterns to that of the original tumor tissue. Our results indicated that this patient-derived xenograft model and cell line would be useful resources for osteosarcoma research.

UR - http://www.scopus.com/inward/record.url?scp=85049048275&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049048275&partnerID=8YFLogxK

U2 - 10.1007/s11626-018-0274-2

DO - 10.1007/s11626-018-0274-2

M3 - Article

VL - 54

SP - 528

EP - 536

JO - In Vitro Cellular and Developmental Biology - Animal

JF - In Vitro Cellular and Developmental Biology - Animal

SN - 1071-2690

IS - 7

ER -