Establishment and characterization of PTHrP-producing human pancreatic cancer cell line

Y. Nishi, M. Haji, R. Takayanagi, H. Iguchi, Takao Shimazoe, J. Hirata, H. Nawata

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Human pancreatic ductal cell carcinoma cell line, which can secrete parathyroid hormone-related peptide (PTHrP), designated as KP 4, and its daughter cell lines with different PTHrP-secreting activities, termed KP 4-1 and KP 4-2, have been established in tissue culture. KP 4 cells were able to form tumors in nude mice. The absolute production rate of PTHrP in KP 4-1 was 5 to 10 times higher than that in KP 4-2. Similarly, the level of PTHrP mRNA in KP 4-1 was significantly higher than that in KP 4-2. KP 4-2 cells exhibited more rapid growth than KP 4 and KP 4-2 vitro. Our established cell lines should provide a useful system to study the regulation of PTHrP production and its pathophysiological roles.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalInternational journal of oncology
Volume5
Issue number1
Publication statusPublished - Jan 1 1994

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Parathyroid Hormone-Related Protein
Pancreatic Neoplasms
Cell Line
Pancreatic Ductal Carcinoma
Nude Mice
Messenger RNA
Growth
Neoplasms

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Nishi, Y., Haji, M., Takayanagi, R., Iguchi, H., Shimazoe, T., Hirata, J., & Nawata, H. (1994). Establishment and characterization of PTHrP-producing human pancreatic cancer cell line. International journal of oncology, 5(1), 33-39.

Establishment and characterization of PTHrP-producing human pancreatic cancer cell line. / Nishi, Y.; Haji, M.; Takayanagi, R.; Iguchi, H.; Shimazoe, Takao; Hirata, J.; Nawata, H.

In: International journal of oncology, Vol. 5, No. 1, 01.01.1994, p. 33-39.

Research output: Contribution to journalArticle

Nishi, Y, Haji, M, Takayanagi, R, Iguchi, H, Shimazoe, T, Hirata, J & Nawata, H 1994, 'Establishment and characterization of PTHrP-producing human pancreatic cancer cell line', International journal of oncology, vol. 5, no. 1, pp. 33-39.
Nishi, Y. ; Haji, M. ; Takayanagi, R. ; Iguchi, H. ; Shimazoe, Takao ; Hirata, J. ; Nawata, H. / Establishment and characterization of PTHrP-producing human pancreatic cancer cell line. In: International journal of oncology. 1994 ; Vol. 5, No. 1. pp. 33-39.
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