Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential

Norihiro Sato, Kazuhiro Mizumoto, Kiichiro Beppu, Naoki Maehara, Masahiro Kusumoto, Toshinaga Nabae, Takashi Morisaki, Mitsuo Katano, Masao Tanaka

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Abstract

We present here a new cell line, NOR-P1, established from a metastatic subcutaneous tumor of a patient with pancreatic cancer. The cells show rapid growth in culture with a doubling time of 16 h and high migration activity. Genetic and molecular analyses revealed high telomerase activity and a mutation in the K-ras oncogene. Of particular interest, the cells express markedly elevated mRNA levels of angiogenic factors, vascular endothelial growth factor and platelet-derived growth factor, as well as other tumor growth-related factors. Subcutaneous transplantation of the NOR-P1 cells into nude mice formed solid, hemorrhagic tumors which were histologically diagnosed as adenocarcinoma with dense blood vessels and severe extravasation of blood. Furthermore, when NOR-P1 cell suspension was injected directly into the pancreas of nude mice, the cells grew rapidly to form intra-pancreatic tumors associated with liver metastases and peritoneal dissemination that resulted in cachexia and subsequent death. These properties suggest that NOR-P1 is an aggressive pancreatic cancer cell line with a high metastatic potential and may serve as a useful experimental model for studying tumor angiogenesis and metastasis of pancreatic cancer. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)153-161
Number of pages9
JournalCancer Letters
Volume155
Issue number2
DOIs
Publication statusPublished - Jul 31 2000

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Pancreatic Neoplasms
Cell Line
Neoplasms
Nude Mice
Neoplasm Metastasis
Cachexia
ras Genes
Angiogenesis Inducing Agents
Telomerase
Platelet-Derived Growth Factor
Vascular Endothelial Growth Factor A
Blood Vessels
Molecular Biology
Pancreas
Intercellular Signaling Peptides and Proteins
Suspensions
Adenocarcinoma
Theoretical Models
Transplantation
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential. / Sato, Norihiro; Mizumoto, Kazuhiro; Beppu, Kiichiro; Maehara, Naoki; Kusumoto, Masahiro; Nabae, Toshinaga; Morisaki, Takashi; Katano, Mitsuo; Tanaka, Masao.

In: Cancer Letters, Vol. 155, No. 2, 31.07.2000, p. 153-161.

Research output: Contribution to journalArticle

Sato, N, Mizumoto, K, Beppu, K, Maehara, N, Kusumoto, M, Nabae, T, Morisaki, T, Katano, M & Tanaka, M 2000, 'Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential', Cancer Letters, vol. 155, no. 2, pp. 153-161. https://doi.org/10.1016/S0304-3835(00)00421-3
Sato, Norihiro ; Mizumoto, Kazuhiro ; Beppu, Kiichiro ; Maehara, Naoki ; Kusumoto, Masahiro ; Nabae, Toshinaga ; Morisaki, Takashi ; Katano, Mitsuo ; Tanaka, Masao. / Establishment of a new human pancreatic cancer cell line, NOR-P1, with high angiogenic activity and metastatic potential. In: Cancer Letters. 2000 ; Vol. 155, No. 2. pp. 153-161.
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