Establishment of a novel method of screening anti-Allergic lactic acid bacteria

Keishi Kadooka, Asuka Imahayashi, Ayaka Koiso, Makiko Yamashita, Kiichiro Teruya, Takashi Matsumoto, Takanori Hasegawa, Fumiki Morimatsu, Yoshinori Katakura

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this study, we attempted to establish a novel method of screening anti-allergic lactic acid bacteria (LAB). We cloned the human histidine decarboxylase (HDC) promoter into the promoter-less pPhi-Yellow-RPL-dest1 vector and established KU812F cells transduced with this vector (pHDCp-Phi-Yellow/ KU812F). After adding LAB to these cells, the change in fluorescence intensity was monitored by flow cytometry. After screening, we identified several LAB strains that downregulated HDC promoter activity. Functional analysis of these LAB strains indicated that two LAB strains inhibited histamine release from KU812F cells, indicating that this assay system can be used to screen for anti-allergic LAB in a high-throughput manner.

Original languageEnglish
Pages (from-to)1016-1018
Number of pages3
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number5
DOIs
Publication statusPublished - Jun 6 2011

Fingerprint

Anti-Allergic Agents
Lactic Acid
Bacteria
Screening
Histidine Decarboxylase
Functional analysis
Flow cytometry
Histamine Release
Histamine
Assays
Flow Cytometry
Down-Regulation
Fluorescence
Throughput

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Establishment of a novel method of screening anti-Allergic lactic acid bacteria. / Kadooka, Keishi; Imahayashi, Asuka; Koiso, Ayaka; Yamashita, Makiko; Teruya, Kiichiro; Matsumoto, Takashi; Hasegawa, Takanori; Morimatsu, Fumiki; Katakura, Yoshinori.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 5, 06.06.2011, p. 1016-1018.

Research output: Contribution to journalArticle

Kadooka, K, Imahayashi, A, Koiso, A, Yamashita, M, Teruya, K, Matsumoto, T, Hasegawa, T, Morimatsu, F & Katakura, Y 2011, 'Establishment of a novel method of screening anti-Allergic lactic acid bacteria' Bioscience, Biotechnology and Biochemistry, vol. 75, no. 5, pp. 1016-1018. https://doi.org/10.1271/bbb.110069
Kadooka, Keishi ; Imahayashi, Asuka ; Koiso, Ayaka ; Yamashita, Makiko ; Teruya, Kiichiro ; Matsumoto, Takashi ; Hasegawa, Takanori ; Morimatsu, Fumiki ; Katakura, Yoshinori. / Establishment of a novel method of screening anti-Allergic lactic acid bacteria. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 5. pp. 1016-1018.
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