TY - JOUR
T1 - Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell
AU - Xu, Jian
AU - Mon, Hiroaki
AU - Kusakabe, Takahiro
AU - Li, Zhiqing
AU - Zhu, Li
AU - Iiyama, Kazuhiro
AU - Masuda, Atsushi
AU - Mitsudome, Takumi
AU - Lee, Jae Man
N1 - Funding Information:
Acknowledgments This work was supported in part by grants KAKENHI nos. 22248003, 22248004, and 23580077 from the Japan Society for the Promotion of Science. The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.
PY - 2013/12
Y1 - 2013/12
N2 - The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAimediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.
AB - The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAimediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.
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U2 - 10.1007/s00253-013-5279-x
DO - 10.1007/s00253-013-5279-x
M3 - Article
C2 - 24081323
AN - SCOPUS:84892140594
SN - 0175-7598
VL - 97
SP - 10435
EP - 10444
JO - Applied Microbiology and Biotechnology
JF - Applied Microbiology and Biotechnology
IS - 24
ER -