Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell

Jian Xu, Hiroaki Mon, Takahiro Kusakabe, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Atsushi Masuda, Takumi Mitsudome, Jae Man Lee

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The double-stranded RNA (dsRNA) mediated RNA interference (RNAi) is widely employed in silkworm and its tissue-derived cell lines for gene function analysis. Baculovirus expression vector system (BEVS) has an advantage for large-scale protein expression. Previously, combining these useful tools, we improved traditional AcMNPV-Sf9 BEVS to produce modified target glycoproteins, where the ectopic expression of Caenorhabditis elegans systemic RNAi defective-1 (SID-1) was found to be valuable for soaking RNAi. In current study, we applied CeSID-1 protein to a Bombyx mori NPV (BmNPV)-hypersensitive Bme21 cell line and investigated its properties both in soaking RNAi ability and recombinant protein expression. The soaking RNAimediated suppression in the Bme21 cell enables us to produce modified glycoproteins of interest in BmNPV-Bme21 BEVS.

Original languageEnglish
Pages (from-to)10435-10444
Number of pages10
JournalApplied Microbiology and Biotechnology
Volume97
Issue number24
DOIs
Publication statusPublished - Dec 2013

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Fingerprint Dive into the research topics of 'Establishment of a soaking RNA interference and Bombyx mori nucleopolyhedrovirus (BmNPV)-hypersensitive cell line using Bme21 cell'. Together they form a unique fingerprint.

Cite this