Establishment of ameloblastoma cell line, AM-1

Hidemitsu Harada, Takeshi Mitsuyasu, Norifumi Nakamura, Yoshinori Higuchi, Kuniaki Toyoshima, Akiyoshi Taniguchi, Shigeru Yasumoto

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Abstract

Ameloblastomas are slowly growing, locally invasive neoplasms with a potentially destructive behaviour. The molecular mechanisms that regulate the cell growth and invasion of ameloblastoma cells are unknown. Because ameloblastoma cells placed in culture have a very limited lifespan, the establishment of immortalized clones of ameloblastoma cells would aid its study. We produced an immortalized ameloblastoma cell line (AM-1) using human papillomavirus type-16. This cell line maintains epithelial cell morphology and expresses cytokeratins K8, K14, K18, K19. Furthermore, bcl-2 protein, which prevents apoptosis, is expressed. We investigated the behaviour of these cells on a collagen matrix in vitro. These cells grew in a monolayer over foci of collagen degradation and could invade the collagen gel at such sites. Since the behavior of cell line AM-1 mimics the behavior of ameloblastoma in vivo, it may be a valuable model for the study of these neoplasms.

Original languageEnglish
Pages (from-to)207-212
Number of pages6
JournalJournal of Oral Pathology and Medicine
Volume27
Issue number5
Publication statusPublished - May 1 1998

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All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Oral Surgery
  • Otorhinolaryngology
  • Cancer Research
  • Periodontics

Cite this

Harada, H., Mitsuyasu, T., Nakamura, N., Higuchi, Y., Toyoshima, K., Taniguchi, A., & Yasumoto, S. (1998). Establishment of ameloblastoma cell line, AM-1. Journal of Oral Pathology and Medicine, 27(5), 207-212.