Establishment of an embryonic stem (ES) cell line derived from a non-obese diabetic (NOD) mouse: In vivo differentiation into lymphocytes and potential for germ line transmission

Seiho Nagafuchi, Hitoshi Katsuta, Kazuhiko Kogawa, Tomoyuki Akashi, Shiori Kondo, Yumiko Sakai, Tadasuke Tsukiyama, Daisuke Kitamura, Yoshiyuki Niho, Takeshi Watanabe

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

A non-obese diabetic (NOD) mouse-derived embryonic stem (ES) cell line has been stably maintained in an undifferentiated state with a characteristic ES cell-like morphology, expressing the stem cell marker alkaline phosphatase, and displaying a normal diploid karyotype. After injecting the NOD-ES cells into blastocysts, chimeric mice were obtained. Small but significant numbers of lymphocytes expressed the NOD-derived MHC allele. When a chimeric mouse was mated with C57BL/6 mice, an agouti mouse was obtained, having the NOD-derived H-2 I-A(β)(g7) haplotype. Thus, an NOD-ES cell line which can differentiate into lymphocytes with potential for germ line transmission was successfully established. Copyright (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)101-104
Number of pages4
JournalFEBS Letters
Volume455
Issue number1-2
DOIs
Publication statusPublished - Jul 16 1999

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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