Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Jian Xu; Kaito Yoshimura; Hiroaki Mon; Zhiqing Li; Li Zhu; Kazuhiro Iiyama; Takahiro Kusakabe; Jae Man Lee

doi:10.1007/s12033-013-9694-0

Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Jae Man Lee

Agricultural Bioresource Sciences

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

Original language	English
Pages (from-to)	193-198
Number of pages	6
Journal	Molecular Biotechnology
Volume	56
Issue number	3
DOIs	https://doi.org/10.1007/s12033-013-9694-0
Publication status	Published - Mar 2014

All Science Journal Classification (ASJC) codes

Biotechnology
Bioengineering
Biochemistry
Applied Microbiology and Biotechnology
Molecular Biology

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title = "Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells",

abstract = "The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.",

author = "Jian Xu and Kaito Yoshimura and Hiroaki Mon and Zhiqing Li and Li Zhu and Kazuhiro Iiyama and Takahiro Kusakabe and Lee, {Jae Man}",

note = "Funding Information: Acknowledgments The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.",

year = "2014",

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doi = "10.1007/s12033-013-9694-0",

language = "English",

volume = "56",

pages = "193--198",

journal = "Molecular Biotechnology",

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T1 - Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

AU - Xu, Jian

AU - Yoshimura, Kaito

AU - Mon, Hiroaki

AU - Li, Zhiqing

AU - Zhu, Li

AU - Iiyama, Kazuhiro

AU - Kusakabe, Takahiro

AU - Lee, Jae Man

N1 - Funding Information: Acknowledgments The cost of publication was supported in part by the Research Grant for Young Investigators of Faculty of Agriculture, Kyushu University.

PY - 2014/3

Y1 - 2014/3

N2 - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

AB - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

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Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Abstract

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