Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

Jian Xu, Kaito Yoshimura, Hiroaki Mon, Zhiqing Li, Li Zhu, Kazuhiro Iiyama, Takahiro Kusakabe, Man Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

Original languageEnglish
Pages (from-to)193-198
Number of pages6
JournalMolecular Biotechnology
Volume56
Issue number3
DOIs
Publication statusPublished - Mar 1 2014

Fingerprint

Bombyx
Caenorhabditis elegans
RNA Interference
RNA
Cultured Cells
DNA
Double-Stranded RNA
Plasmids
Transformed Cell Line
Toxicity
Genes
Cells
Cytosol
Membranes
Cell Membrane
Costs and Cost Analysis
Costs

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology

Cite this

Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells. / Xu, Jian; Yoshimura, Kaito; Mon, Hiroaki; Li, Zhiqing; Zhu, Li; Iiyama, Kazuhiro; Kusakabe, Takahiro; Lee, Man.

In: Molecular Biotechnology, Vol. 56, No. 3, 01.03.2014, p. 193-198.

Research output: Contribution to journalArticle

@article{65ecb8e47f43425c89eb032856c1eec1,
title = "Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells",
abstract = "The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.",
author = "Jian Xu and Kaito Yoshimura and Hiroaki Mon and Zhiqing Li and Li Zhu and Kazuhiro Iiyama and Takahiro Kusakabe and Man Lee",
year = "2014",
month = "3",
day = "1",
doi = "10.1007/s12033-013-9694-0",
language = "English",
volume = "56",
pages = "193--198",
journal = "Molecular Biotechnology",
issn = "1073-6085",
publisher = "Humana Press",
number = "3",

}

TY - JOUR

T1 - Establishment of Caenorhabditis elegans SID-1-dependent DNA delivery system in cultured silkworm cells

AU - Xu, Jian

AU - Yoshimura, Kaito

AU - Mon, Hiroaki

AU - Li, Zhiqing

AU - Zhu, Li

AU - Iiyama, Kazuhiro

AU - Kusakabe, Takahiro

AU - Lee, Man

PY - 2014/3/1

Y1 - 2014/3/1

N2 - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

AB - The systemic RNA interference defective-1 (SID-1) can transport double-stranded RNA (dsRNA) into cytosol across the cytoplasmic membrane. We report here that ectopic expression of Caenorhabditis elegans SID-1 allows BmN4 cells to import extracellular plasmid dsDNA into cells via the direct soaking method. Interestingly, BmN4-SID1 cells incorporate dsRNA and plasmid DNA simultaneously. Furthermore, the ectopic SID-1 allows us to establish a stably transformed cell line by the simple soaking method. Our results provide an alternative method for silkworm gene function analysis with low cost and low cell toxicity.

UR - http://www.scopus.com/inward/record.url?scp=84896722607&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84896722607&partnerID=8YFLogxK

U2 - 10.1007/s12033-013-9694-0

DO - 10.1007/s12033-013-9694-0

M3 - Article

C2 - 23979877

AN - SCOPUS:84896722607

VL - 56

SP - 193

EP - 198

JO - Molecular Biotechnology

JF - Molecular Biotechnology

SN - 1073-6085

IS - 3

ER -