Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells

A. Suzuki; K. Oyama; K. Fukao; H. Nakauchi; H. Taniguchi

doi:10.3727/000000002783985765

Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells

A. Suzuki, K. Oyama, K. Fukao, H. Nakauchi, H. Taniguchi

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as α and β cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.

Original language	English
Pages (from-to)	451-453
Number of pages	3
Journal	Cell Transplantation
Volume	11
Issue number	5
DOIs	https://doi.org/10.3727/000000002783985765
Publication status	Published - 2002
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biomedical Engineering
Cell Biology
Transplantation

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abstract = "Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as α and β cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.",

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AU - Suzuki, A.

AU - Oyama, K.

AU - Fukao, K.

AU - Nakauchi, H.

AU - Taniguchi, H.

PY - 2002

Y1 - 2002

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AB - Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as α and β cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.

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Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells

Abstract

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