Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells

A. Suzuki, K. Oyama, K. Fukao, H. Nakauchi, H. Taniguchi

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Pluripotent stem cells found in a number of organs are usually in small cell populations. However, under adaptive stimulation, they enter the stage of growth and differentiation to compensate for the loss of differentiated cells. To analyze stem cell potential precisely, the exclusion of other differentiated cells and a clonal assay system are strongly required. In this study, we established a colony-forming assay system for pancreatic stem/progenitor cells in vitro. In this culture condition, they received signals for growth and differentiation, and formed clonal colonies including pancreatic endocrine-lineage cells, such as α and β cells. By combining this culture system with flow cytometric cell sorting, pancreatic stem/progenitor cells will be enriched, and their potential can be analyzed precisely in single cell-based experiments.

Original languageEnglish
Pages (from-to)451-453
Number of pages3
JournalCell Transplantation
Volume11
Issue number5
Publication statusPublished - Oct 16 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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    Suzuki, A., Oyama, K., Fukao, K., Nakauchi, H., & Taniguchi, H. (2002). Establishment of clonal colony-forming assay system for pancreatic stem/progenitor cells. Cell Transplantation, 11(5), 451-453.