Establishment of dental epithelial cell line (HAT-7) and the cell differentiation dependent on Notch signaling pathway

Shintaro Kawano, Takahiko Morotomi, Takashi Toyono, Norifumi Nakamura, Takashi Uchida, Masamichi Ohishi, Kuniaki Toyoshima, Hidemitsu Harada

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78 Citations (Scopus)


Rat incisors grow continuously throughout life. Producing a variety of dental epithelial cells is performed by stem cells located in the cervical loop of the incisor apex. To study the mechanisms for cell differentiation, we established a dental epithelial cell line (HAT-7) originating from a cervical loop epithelium of a rat incisor. Immunochemical studies showed that HAT-7 produced the cells expressing amelogenin, ameloblastin, or alkaline phosphatase (ALP). To illustrate a role of Notch signaling in the determinant of the cell fate, we examined expression patterns of Notch1 and Jagged1 in HAT-7 density dependently. At lower cell density, Notch1- or Jagged1-expressing cells were not seen. However, when they were fully confluent, cells began to express Notch1 or Jagged1 strongly. Some ALP-positive cells were almost consistent with Notch1-expressing cells but not Jagged1-expressing cells. These results suggested that the determinant of direction of differentiation was associated with Notch signaling pathway.

Original languageEnglish
Pages (from-to)409-412
Number of pages4
JournalConnective Tissue Research
Issue number2-3
Publication statusPublished - 2002

All Science Journal Classification (ASJC) codes

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology


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