TY - JOUR
T1 - Establishment of dental epithelial cell line (HAT-7) and the cell differentiation dependent on Notch signaling pathway
AU - Kawano, Shintaro
AU - Morotomi, Takahiko
AU - Toyono, Takashi
AU - Nakamura, Norifumi
AU - Uchida, Takashi
AU - Ohishi, Masamichi
AU - Toyoshima, Kuniaki
AU - Harada, Hidemitsu
N1 - Funding Information:
This work was partly funded by grants from the Ministry of Education, Science, Sports, and Culture and the Kato Memorial Bioscience Foundation in Japan.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2002
Y1 - 2002
N2 - Rat incisors grow continuously throughout life. Producing a variety of dental epithelial cells is performed by stem cells located in the cervical loop of the incisor apex. To study the mechanisms for cell differentiation, we established a dental epithelial cell line (HAT-7) originating from a cervical loop epithelium of a rat incisor. Immunochemical studies showed that HAT-7 produced the cells expressing amelogenin, ameloblastin, or alkaline phosphatase (ALP). To illustrate a role of Notch signaling in the determinant of the cell fate, we examined expression patterns of Notch1 and Jagged1 in HAT-7 density dependently. At lower cell density, Notch1- or Jagged1-expressing cells were not seen. However, when they were fully confluent, cells began to express Notch1 or Jagged1 strongly. Some ALP-positive cells were almost consistent with Notch1-expressing cells but not Jagged1-expressing cells. These results suggested that the determinant of direction of differentiation was associated with Notch signaling pathway.
AB - Rat incisors grow continuously throughout life. Producing a variety of dental epithelial cells is performed by stem cells located in the cervical loop of the incisor apex. To study the mechanisms for cell differentiation, we established a dental epithelial cell line (HAT-7) originating from a cervical loop epithelium of a rat incisor. Immunochemical studies showed that HAT-7 produced the cells expressing amelogenin, ameloblastin, or alkaline phosphatase (ALP). To illustrate a role of Notch signaling in the determinant of the cell fate, we examined expression patterns of Notch1 and Jagged1 in HAT-7 density dependently. At lower cell density, Notch1- or Jagged1-expressing cells were not seen. However, when they were fully confluent, cells began to express Notch1 or Jagged1 strongly. Some ALP-positive cells were almost consistent with Notch1-expressing cells but not Jagged1-expressing cells. These results suggested that the determinant of direction of differentiation was associated with Notch signaling pathway.
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U2 - 10.1080/03008200290000637
DO - 10.1080/03008200290000637
M3 - Article
C2 - 12489191
AN - SCOPUS:0035996549
VL - 43
SP - 409
EP - 412
JO - Connective Tissue Research
JF - Connective Tissue Research
SN - 0300-8207
IS - 2-3
ER -