Establishment of estrogen receptor 1 (ESR1)-knockout medaka

ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes

Saki Tohyama, Yukiko Ogino, Anke Lange, Taijun Myosho, Tohru Kobayashi, Yu Hirano, Gen Yamada, Tomomi Sato, Norihisa Tatarazako, Charles R. Tyler, Taisen Iguchi, Shinichi Miyagawa

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Estrogens play fundamental roles in regulating reproductive activities and they act through estrogen receptor (ESR) in all vertebrates. Most vertebrates have two ESR subtypes (ESR1 and ESR2), whereas teleost fish have at least three (Esr1, Esr2a and Esr2b). Intricate functionalization has been suggested among the Esr subtypes, but to date, distinct roles of Esr have been characterized in only a limited number of species. Study of loss-of-function in animal models is a powerful tool for application to understanding vertebrate reproductive biology. In the current study, we established esr1 knockout (KO) medaka using a TALEN approach and examined the effects of Esr1 ablation. Unexpectedly, esr1 KO medaka did not show any significant defects in their gonadal development or in their sexual characteristics. Neither male or female esr1 KO medaka exhibited any significant changes in sexual differentiation or reproductive activity compared with wild type controls. Interestingly, however, estrogen-induced vitellogenin gene expression, an estrogen-responsive biomarker in fish, was limited in the liver of esr1 KO males. Our findings, in contrast to mammals, indicate that Esr1 is dispensable for normal development and reproduction in medaka. We thus provide an evidence for estrogen receptor functionalization between mammals and fish. Our findings will also benefit interpretation of studies into the toxicological effects of estrogenic chemicals in fish.

Original languageEnglish
Pages (from-to)552-561
Number of pages10
JournalDevelopment Growth and Differentiation
Volume59
Issue number6
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Oryzias
Sexual Development
Estrogen Receptor alpha
Reproduction
Fishes
Estrogen Receptors
Vertebrates
Estrogens
Mammals
Vitellogenins
Sex Differentiation
Toxicology
Animal Models
Biomarkers
Gene Expression
Liver

All Science Journal Classification (ASJC) codes

  • Developmental Biology
  • Cell Biology

Cite this

Establishment of estrogen receptor 1 (ESR1)-knockout medaka : ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes. / Tohyama, Saki; Ogino, Yukiko; Lange, Anke; Myosho, Taijun; Kobayashi, Tohru; Hirano, Yu; Yamada, Gen; Sato, Tomomi; Tatarazako, Norihisa; Tyler, Charles R.; Iguchi, Taisen; Miyagawa, Shinichi.

In: Development Growth and Differentiation, Vol. 59, No. 6, 01.08.2017, p. 552-561.

Research output: Contribution to journalArticle

Tohyama, S, Ogino, Y, Lange, A, Myosho, T, Kobayashi, T, Hirano, Y, Yamada, G, Sato, T, Tatarazako, N, Tyler, CR, Iguchi, T & Miyagawa, S 2017, 'Establishment of estrogen receptor 1 (ESR1)-knockout medaka: ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes', Development Growth and Differentiation, vol. 59, no. 6, pp. 552-561. https://doi.org/10.1111/dgd.12386
Tohyama, Saki ; Ogino, Yukiko ; Lange, Anke ; Myosho, Taijun ; Kobayashi, Tohru ; Hirano, Yu ; Yamada, Gen ; Sato, Tomomi ; Tatarazako, Norihisa ; Tyler, Charles R. ; Iguchi, Taisen ; Miyagawa, Shinichi. / Establishment of estrogen receptor 1 (ESR1)-knockout medaka : ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes. In: Development Growth and Differentiation. 2017 ; Vol. 59, No. 6. pp. 552-561.
@article{fa7fe9e5071144d293f63b9fbc70481b,
title = "Establishment of estrogen receptor 1 (ESR1)-knockout medaka: ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes",
abstract = "Estrogens play fundamental roles in regulating reproductive activities and they act through estrogen receptor (ESR) in all vertebrates. Most vertebrates have two ESR subtypes (ESR1 and ESR2), whereas teleost fish have at least three (Esr1, Esr2a and Esr2b). Intricate functionalization has been suggested among the Esr subtypes, but to date, distinct roles of Esr have been characterized in only a limited number of species. Study of loss-of-function in animal models is a powerful tool for application to understanding vertebrate reproductive biology. In the current study, we established esr1 knockout (KO) medaka using a TALEN approach and examined the effects of Esr1 ablation. Unexpectedly, esr1 KO medaka did not show any significant defects in their gonadal development or in their sexual characteristics. Neither male or female esr1 KO medaka exhibited any significant changes in sexual differentiation or reproductive activity compared with wild type controls. Interestingly, however, estrogen-induced vitellogenin gene expression, an estrogen-responsive biomarker in fish, was limited in the liver of esr1 KO males. Our findings, in contrast to mammals, indicate that Esr1 is dispensable for normal development and reproduction in medaka. We thus provide an evidence for estrogen receptor functionalization between mammals and fish. Our findings will also benefit interpretation of studies into the toxicological effects of estrogenic chemicals in fish.",
author = "Saki Tohyama and Yukiko Ogino and Anke Lange and Taijun Myosho and Tohru Kobayashi and Yu Hirano and Gen Yamada and Tomomi Sato and Norihisa Tatarazako and Tyler, {Charles R.} and Taisen Iguchi and Shinichi Miyagawa",
year = "2017",
month = "8",
day = "1",
doi = "10.1111/dgd.12386",
language = "English",
volume = "59",
pages = "552--561",
journal = "Development Growth and Differentiation",
issn = "0012-1592",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Establishment of estrogen receptor 1 (ESR1)-knockout medaka

T2 - ESR1 is dispensable for sexual development and reproduction in medaka, Oryzias latipes

AU - Tohyama, Saki

AU - Ogino, Yukiko

AU - Lange, Anke

AU - Myosho, Taijun

AU - Kobayashi, Tohru

AU - Hirano, Yu

AU - Yamada, Gen

AU - Sato, Tomomi

AU - Tatarazako, Norihisa

AU - Tyler, Charles R.

AU - Iguchi, Taisen

AU - Miyagawa, Shinichi

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Estrogens play fundamental roles in regulating reproductive activities and they act through estrogen receptor (ESR) in all vertebrates. Most vertebrates have two ESR subtypes (ESR1 and ESR2), whereas teleost fish have at least three (Esr1, Esr2a and Esr2b). Intricate functionalization has been suggested among the Esr subtypes, but to date, distinct roles of Esr have been characterized in only a limited number of species. Study of loss-of-function in animal models is a powerful tool for application to understanding vertebrate reproductive biology. In the current study, we established esr1 knockout (KO) medaka using a TALEN approach and examined the effects of Esr1 ablation. Unexpectedly, esr1 KO medaka did not show any significant defects in their gonadal development or in their sexual characteristics. Neither male or female esr1 KO medaka exhibited any significant changes in sexual differentiation or reproductive activity compared with wild type controls. Interestingly, however, estrogen-induced vitellogenin gene expression, an estrogen-responsive biomarker in fish, was limited in the liver of esr1 KO males. Our findings, in contrast to mammals, indicate that Esr1 is dispensable for normal development and reproduction in medaka. We thus provide an evidence for estrogen receptor functionalization between mammals and fish. Our findings will also benefit interpretation of studies into the toxicological effects of estrogenic chemicals in fish.

AB - Estrogens play fundamental roles in regulating reproductive activities and they act through estrogen receptor (ESR) in all vertebrates. Most vertebrates have two ESR subtypes (ESR1 and ESR2), whereas teleost fish have at least three (Esr1, Esr2a and Esr2b). Intricate functionalization has been suggested among the Esr subtypes, but to date, distinct roles of Esr have been characterized in only a limited number of species. Study of loss-of-function in animal models is a powerful tool for application to understanding vertebrate reproductive biology. In the current study, we established esr1 knockout (KO) medaka using a TALEN approach and examined the effects of Esr1 ablation. Unexpectedly, esr1 KO medaka did not show any significant defects in their gonadal development or in their sexual characteristics. Neither male or female esr1 KO medaka exhibited any significant changes in sexual differentiation or reproductive activity compared with wild type controls. Interestingly, however, estrogen-induced vitellogenin gene expression, an estrogen-responsive biomarker in fish, was limited in the liver of esr1 KO males. Our findings, in contrast to mammals, indicate that Esr1 is dispensable for normal development and reproduction in medaka. We thus provide an evidence for estrogen receptor functionalization between mammals and fish. Our findings will also benefit interpretation of studies into the toxicological effects of estrogenic chemicals in fish.

UR - http://www.scopus.com/inward/record.url?scp=85027312510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85027312510&partnerID=8YFLogxK

U2 - 10.1111/dgd.12386

DO - 10.1111/dgd.12386

M3 - Article

VL - 59

SP - 552

EP - 561

JO - Development Growth and Differentiation

JF - Development Growth and Differentiation

SN - 0012-1592

IS - 6

ER -