Establishment of portable immunoassay system for early diagnosis

Toshihiro Kasama, Yutaka Hasegawa, Hiroyuki Matsumoto, Haruyasu Kondo, Tsutomu Ozawa, Noritada Kaji, Manabu Tokeshi, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have developed immunopillar devices for rapid and easy-to-use immunoassay with pM-fM detection sensitivity, but long total assay time (sample-in-answer-out) and the compatibility with a portable detection system have still remained as major problems toward the point-of-care testing (POCT). We report here next generation immunopillar devices and portable detection system suitable for them. Thin structure of those devices allowed us to wash nonspecifically bound antigens and fluorescent-labeled secondary antibodies in a minute, resulting in the dramatic reduction in the total assay time. In addition, by using the portable detection system, we gained the concentrations of C-reactive protein in human sera while preserving pM detection sensitivity. The total assay time was 20 minutes per sample. Our immunoassay system possesses the potential for use in POCT.

Original languageEnglish
Title of host publication2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
PublisherIEEE Computer Society
ISBN (Print)9781479915293
DOIs
Publication statusPublished - 2013
Externally publishedYes
Event2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 - Nagoya, Japan
Duration: Nov 10 2013Nov 13 2013

Publication series

Name2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013

Other

Other2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013
CountryJapan
CityNagoya
Period11/10/1311/13/13

All Science Journal Classification (ASJC) codes

  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Establishment of portable immunoassay system for early diagnosis'. Together they form a unique fingerprint.

  • Cite this

    Kasama, T., Hasegawa, Y., Matsumoto, H., Kondo, H., Ozawa, T., Kaji, N., Tokeshi, M., & Baba, Y. (2013). Establishment of portable immunoassay system for early diagnosis. In 2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013 [6710451] (2013 International Symposium on Micro-NanoMechatronics and Human Science, MHS 2013). IEEE Computer Society. https://doi.org/10.1109/MHS.2013.6710451