Establishment of protoplast culture of Solanum sisymbriifolium

Naoki Oda, Shiro Isshiki, Takeshi Sadohara, Yukio Ozaki, Hiroshi Okubo

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The optimal culture conditions were studied for plant regeneration from mesophyll protoplasts of Solanum sisymbriifolium,. Aseptic seedlings of S. sisymbriifolium were used as a material for protoplast culture. Many viable protoplasts were isolated by incubating leaf slices in an enzyme solution containing 0.25% Meiserase and 0.05% Macerozyme for 16h at 25°C without shaking. Protoplast density of 5.0 × 104/ml in Kao medium containing 5.0mg/l NAA, 1.0mg/1 2,4-D and 1.0mg/1 BA was optimal for colony formation. Most colonies formed when protoplasts were cultured at 25°C after initial culture at 30°C for one week. On the MS agar medium with 1.0mg/1 zeatin, 38.5% of protoplast-derived calli differentiated shoots. These shoots rooted on 1/2 MS medium with 5.0mg/l sucrose and 2.5g/l gellan gum, and developed into whole plants.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume51
Issue number1
Publication statusPublished - Feb 2006

Fingerprint

Solanum sisymbriifolium
Solanum
Protoplasts
protoplasts
Zeatin
gellan gum
2,4-Dichlorophenoxyacetic Acid
shoots
Bony Callus
zeatin
Seedlings
naphthaleneacetic acid
mesophyll
2,4-D
Agar
Sucrose
Regeneration
callus
agar
sucrose

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Establishment of protoplast culture of Solanum sisymbriifolium. / Oda, Naoki; Isshiki, Shiro; Sadohara, Takeshi; Ozaki, Yukio; Okubo, Hiroshi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 51, No. 1, 02.2006, p. 63-66.

Research output: Contribution to journalArticle

Oda, Naoki ; Isshiki, Shiro ; Sadohara, Takeshi ; Ozaki, Yukio ; Okubo, Hiroshi. / Establishment of protoplast culture of Solanum sisymbriifolium. In: Journal of the Faculty of Agriculture, Kyushu University. 2006 ; Vol. 51, No. 1. pp. 63-66.
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