TY - JOUR
T1 - Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori
AU - Karasaki, Noriko
AU - Mon, Hiroaki
AU - Takahashi, Masateru
AU - Lee, Jae Man
AU - Koga, Katsumi
AU - Kawaguchi, Yutaka
AU - Kusakabe, Takahiro
N1 - Funding Information:
Acknowledgments This work was supported in part by the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), by a grant from the Ministry of Agriculture, Forestry and Fisheries of Japan (Integrated research project for plant, insect and animal using genome technology INSECT-1201), and by grants-in-aid no.
PY - 2009/4
Y1 - 2009/4
N2 - Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
AB - Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.
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U2 - 10.1007/s10529-008-9898-8
DO - 10.1007/s10529-008-9898-8
M3 - Article
C2 - 19066730
AN - SCOPUS:61449252439
SN - 0141-5492
VL - 31
SP - 495
EP - 500
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 4
ER -