Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori

Noriko Karasaki, Hiroaki Mon, Masateru Takahashi, Man Lee, Katsumi Koga, Yutaka Kawaguchi, Takahiro Kusakabe

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Tetracycline-inducible gene expression (Tet-on) system has become one of the first choices for the control of transgenes expression in mammal and drosophila. However, the Tet-on systems that have been established in mammalian system or tuned into drosophila do not function in the silkworm, Bombyx mori. To construct a functional Tet-on system in B. mori, we modified rtTA by introducing a transcription activation domain of immediate-early gene 1 of Autographa californica nuclear polyhedrosis virus and nuclear localization signal of SV40 large T-antigen. The modified rtTA can activate the transcription from 9 × tetO promoter in the silkworm cells up to 250-fold in the presence of doxycycline.

Original languageEnglish
Pages (from-to)495-500
Number of pages6
JournalBiotechnology letters
Volume31
Issue number4
DOIs
Publication statusPublished - Apr 1 2009

Fingerprint

Bombyx
Transcription
Tetracycline
Gene expression
Gene Expression
Mammals
Doxycycline
Viral Tumor Antigens
Antigens
Viruses
Drosophila
Genes
Chemical activation
Nucleopolyhedrovirus
Polyomavirus Transforming Antigens
Nuclear Localization Signals
Immediate-Early Genes
Transgenes
Transcriptional Activation

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori. / Karasaki, Noriko; Mon, Hiroaki; Takahashi, Masateru; Lee, Man; Koga, Katsumi; Kawaguchi, Yutaka; Kusakabe, Takahiro.

In: Biotechnology letters, Vol. 31, No. 4, 01.04.2009, p. 495-500.

Research output: Contribution to journalArticle

Karasaki, Noriko ; Mon, Hiroaki ; Takahashi, Masateru ; Lee, Man ; Koga, Katsumi ; Kawaguchi, Yutaka ; Kusakabe, Takahiro. / Establishment of tetracycline-inducible gene expression systems in the silkworm, Bombyx mori. In: Biotechnology letters. 2009 ; Vol. 31, No. 4. pp. 495-500.
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