Esterification in organic solvents: Selection of hydrolases and effects of reaction conditions

Takuo Kawamoto, Kenji Sonomoto, Atsuo Tanaka

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)


Fifty different hydrolases were screened for retention of high esterification activity in an organic solvent with citronellol as substrate. Although 22 hydrolases were very active as catalysts in the organic solvent, lipase from Candida cylindracea (lipase OF 360) was selected for further examination of the effects of reaction conditions on enzyme activity, with regard to catalyst availability and activity retention after immobilization. When the enzyme was entrapped in hydrophobic polyurethane gels, water-saturated isooctane was found to be the most suitable solvent, whereas polar solvents caused reversible catalyst inactivation. Entrapment significantly enhanced the operational stability of the lipase in the organic solvent.

Original languageEnglish
Pages (from-to)137-145
Number of pages9
JournalBiocatalysis and Biotransformation
Issue number2
Publication statusPublished - 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Catalysis
  • Biochemistry


Dive into the research topics of 'Esterification in organic solvents: Selection of hydrolases and effects of reaction conditions'. Together they form a unique fingerprint.

Cite this