TY - JOUR
T1 - Esterification in organic solvents
T2 - Selection of hydrolases and effects of reaction conditions
AU - Kawamoto, Takuo
AU - Sonomoto, Kenji
AU - Tanaka, Atsuo
N1 - Funding Information:
We are indebted to Mr S. Koshiro, Toyo Tire and Rubber Industry Co., and to Mr. T. Iida, Kansai Paint Co., for their generous supply of resin prepolymers used in this study. This work was supported in part by a grant-in-aid for research from the Ministry of Education, Science and Culture, Japan.
PY - 1987
Y1 - 1987
N2 - Fifty different hydrolases were screened for retention of high esterification activity in an organic solvent with citronellol as substrate. Although 22 hydrolases were very active as catalysts in the organic solvent, lipase from Candida cylindracea (lipase OF 360) was selected for further examination of the effects of reaction conditions on enzyme activity, with regard to catalyst availability and activity retention after immobilization. When the enzyme was entrapped in hydrophobic polyurethane gels, water-saturated isooctane was found to be the most suitable solvent, whereas polar solvents caused reversible catalyst inactivation. Entrapment significantly enhanced the operational stability of the lipase in the organic solvent.
AB - Fifty different hydrolases were screened for retention of high esterification activity in an organic solvent with citronellol as substrate. Although 22 hydrolases were very active as catalysts in the organic solvent, lipase from Candida cylindracea (lipase OF 360) was selected for further examination of the effects of reaction conditions on enzyme activity, with regard to catalyst availability and activity retention after immobilization. When the enzyme was entrapped in hydrophobic polyurethane gels, water-saturated isooctane was found to be the most suitable solvent, whereas polar solvents caused reversible catalyst inactivation. Entrapment significantly enhanced the operational stability of the lipase in the organic solvent.
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U2 - 10.3109/10242428709040138
DO - 10.3109/10242428709040138
M3 - Article
AN - SCOPUS:0000868287
SN - 1024-2422
VL - 1
SP - 137
EP - 145
JO - Biocatalysis and Biotransformation
JF - Biocatalysis and Biotransformation
IS - 2
ER -