TY - JOUR
T1 - Estimating age of humans based on telomere shortening
AU - Tsuji, Akiko
AU - Ishiko, Atsushi
AU - Takasaki, Tomoya
AU - Ikeda, Noriaki
PY - 2002/5/23
Y1 - 2002/5/23
N2 - To estimate age using DNA based on telomere shortening, we determined the terminal restriction fragment (TRF) length, as telomere length, using Southern blot analysis of peripheral human blood and blood stains. All blood stains had been stored at room temperature for 5 months. The average TRF length clearly showed a tendency to shortening with aging. The formula for age estimation was based on a correlation between average TRF length and age of the subjects. The estimated age calculated from TRF length widely depends on environmental and genetic factors. However, as long as the DNA is well preserved, use of our method is feasible regardless of age of the subject and can give a rough estimation of age of subjects in forensic samples that carry no morphological information.
AB - To estimate age using DNA based on telomere shortening, we determined the terminal restriction fragment (TRF) length, as telomere length, using Southern blot analysis of peripheral human blood and blood stains. All blood stains had been stored at room temperature for 5 months. The average TRF length clearly showed a tendency to shortening with aging. The formula for age estimation was based on a correlation between average TRF length and age of the subjects. The estimated age calculated from TRF length widely depends on environmental and genetic factors. However, as long as the DNA is well preserved, use of our method is feasible regardless of age of the subject and can give a rough estimation of age of subjects in forensic samples that carry no morphological information.
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U2 - 10.1016/S0379-0738(02)00086-5
DO - 10.1016/S0379-0738(02)00086-5
M3 - Article
C2 - 12062940
AN - SCOPUS:0037161498
VL - 126
SP - 197
EP - 199
JO - Forensic Science International
JF - Forensic Science International
SN - 0379-0738
IS - 3
ER -