Estimating age of humans based on telomere shortening

Akiko Tsuji, Atsushi Ishiko, Tomoya Takasaki, Noriaki Ikeda

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

To estimate age using DNA based on telomere shortening, we determined the terminal restriction fragment (TRF) length, as telomere length, using Southern blot analysis of peripheral human blood and blood stains. All blood stains had been stored at room temperature for 5 months. The average TRF length clearly showed a tendency to shortening with aging. The formula for age estimation was based on a correlation between average TRF length and age of the subjects. The estimated age calculated from TRF length widely depends on environmental and genetic factors. However, as long as the DNA is well preserved, use of our method is feasible regardless of age of the subject and can give a rough estimation of age of subjects in forensic samples that carry no morphological information.

Original languageEnglish
Pages (from-to)197-199
Number of pages3
JournalForensic Science International
Volume126
Issue number3
DOIs
Publication statusPublished - May 23 2002

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All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

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