Estimation of actin and 18S rRNA as internal controls of RNA in hyacinth (Hyacinthus orientalis L.)

Azusa Sato, Kazuyuki Saitou, Hiroshi Okubo

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The potential of different housekeeping genes for their use as internal controls of gene expression under changing environmental conditions was investigated in hyacinth (Hyacinthus orientalis L.) bulbs during storage at low (5°C) and room temperatures (25°C) and culture period at 25°C. Proper annealing temperatures for the expression analysis of genes for actin were between 50 and 63°C, and proper PCR cycles for actin and 18S rRNA were 30 and 25, respectively. The expression of actin increased during cold storage period and unstable during culture, whereas that of 18S rRNA was kept unchanged throughout the storage and culture periods. These results indicated that 18S rRNA was more reliable than actin as an internal control for semi-quantitative RT-PCR of total RNA in hyacinth. This is the first report on the establishment of the internal standard for the studies on expression levels of genes in bulbous plants.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume50
Issue number1
Publication statusPublished - Feb 2005

Fingerprint

Hyacinthus
Hyacinthus orientalis
actin
Actins
ribosomal RNA
RNA
Gene Expression
Polymerase Chain Reaction
Temperature
annealing
Essential Genes
cold storage
bulbs
ambient temperature
storage time
genes
reverse transcriptase polymerase chain reaction
gene expression
environmental factors
temperature

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Estimation of actin and 18S rRNA as internal controls of RNA in hyacinth (Hyacinthus orientalis L.). / Sato, Azusa; Saitou, Kazuyuki; Okubo, Hiroshi.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 50, No. 1, 02.2005, p. 103-108.

Research output: Contribution to journalArticle

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