Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium

Naoki Matsunaga, Kenjiro Kano, Yasuyuki Maki, Toshiaki Dobashi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Dissolved carbon dioxide (dCO2) accumulation is one of the most serious problems in the scale-up of industrial cell culture. To predict the effects of dCO2 stripping in different culture conditions and at different scales, we examined a method of estimation of dCO2 stripping using a model medium. The operational parameters (e.g., sparging and agitation rate) and the size of the bioreactor (working volume: 80 L, 500 L, 2000 L; aspect ratio: 1.0 ∼ 1.6) were varied, and the model medium was prepared by adjusting pH, density, viscosity, surface tension, and buffer conditions. dCO2 stripping efficiency was evaluated using the index kLaCO2, which was defined in accordance with the volumetric oxygen transfer coefficient kLa. The model medium exhibited dCO2 stripping behavior similar to real culture medium in all experimental conditions tested. It is expected that the use of the model medium to estimate dCO2 stripping in real cultures will be valuable for determining the culture conditions in bioreactors in scale-up.

Original languageEnglish
Pages (from-to)419-424
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume107
Issue number4
DOIs
Publication statusPublished - Apr 1 2009
Externally publishedYes

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Bioreactors
Carbon Dioxide
Carbon dioxide
Surface Tension
Viscosity
Culture Media
Buffers
Cell Culture Techniques
Oxygen
Cell culture
Surface tension
Aspect ratio

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium. / Matsunaga, Naoki; Kano, Kenjiro; Maki, Yasuyuki; Dobashi, Toshiaki.

In: Journal of Bioscience and Bioengineering, Vol. 107, No. 4, 01.04.2009, p. 419-424.

Research output: Contribution to journalArticle

Matsunaga, Naoki ; Kano, Kenjiro ; Maki, Yasuyuki ; Dobashi, Toshiaki. / Estimation of dissolved carbon dioxide stripping in a large bioreactor using model medium. In: Journal of Bioscience and Bioengineering. 2009 ; Vol. 107, No. 4. pp. 419-424.
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