Abstract
The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj2Rj3, Rj3, Rj4 and Rj 2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H′ β/H′γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2-genotype and non-Rj, Rj3 or Rj4-genotype cultivars were significantly different. These results suggest that the Rj 2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.
| Original language | English |
|---|---|
| Pages (from-to) | 65-72 |
| Number of pages | 8 |
| Journal | Soil Science and Plant Nutrition |
| Volume | 55 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1 2009 |
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All Science Journal Classification (ASJC) codes
- Soil Science
- Plant Science
Cite this
Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol. / Minami, Mai; Yamakawa, Takeo; Yamamoto, Akihiro; Akao, Shoichiro; Saeki, Yuichi.
In: Soil Science and Plant Nutrition, Vol. 55, No. 1, 01.02.2009, p. 65-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol
AU - Minami, Mai
AU - Yamakawa, Takeo
AU - Yamamoto, Akihiro
AU - Akao, Shoichiro
AU - Saeki, Yuichi
PY - 2009/2/1
Y1 - 2009/2/1
N2 - The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj2Rj3, Rj3, Rj4 and Rj 2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H′ β/H′γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2-genotype and non-Rj, Rj3 or Rj4-genotype cultivars were significantly different. These results suggest that the Rj 2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.
AB - The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj2Rj3, Rj3, Rj4 and Rj 2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H′ β/H′γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2-genotype and non-Rj, Rj3 or Rj4-genotype cultivars were significantly different. These results suggest that the Rj 2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.
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U2 - 10.1111/j.1747-0765.2008.00333.x
DO - 10.1111/j.1747-0765.2008.00333.x
M3 - Article
AN - SCOPUS:59149100437
VL - 55
SP - 65
EP - 72
JO - Soil Science and Plant Nutrition
JF - Soil Science and Plant Nutrition
SN - 0038-0768
IS - 1
ER -