Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol

Mai Minami, Takeo Yamakawa, Akihiro Yamamoto, Shoichiro Akao, Yuichi Saeki

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj2Rj3, Rj3, Rj4 and Rj 2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H′ β/H′γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2-genotype and non-Rj, Rj3 or Rj4-genotype cultivars were significantly different. These results suggest that the Rj 2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalSoil Science and Plant Nutrition
Volume55
Issue number1
DOIs
Publication statusPublished - Feb 2009

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

Fingerprint Dive into the research topics of 'Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol'. Together they form a unique fingerprint.

Cite this