Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol

Mai Minami, Takeo Yamakawa, Akihiro Yamamoto, Shoichiro Akao, Yuichi Saeki

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The nodulation tendency of indigenous soybean bradyrhizobia on Rj-genotype soybean cultivars was investigated using approximately 260 bradyrhizobia isolated from an Andosol with 13 soybean cultivars of five Rj-genotypes (non-Rj, Rj2Rj3, Rj3, Rj4 and Rj 2Rj3Rj4). A dendrogram was constructed based on restriction fragment length polymorphism analysis of the polymerase chain reaction products (PCR-RFLP) of the 16S-23S rDNA internal transcribed spacer (ITS) region. Bradyrhizobium USDA strains were used as a reference. The dendrogram indicated nine clusters based on similarities among the reference strains. The ratio of beta diversity to gamma diversity (H′ β/H′γ), which represents differences in the bradyrhizobial communities by pair-wise comparison between each cultivar, was obtained from Shannon-Wiener diversity indices. The results showed that bradyrhizobial communities among the same Rj-genotype cultivars were similar to each other, whereas bradyrhizobial communities between the Rj 2-genotype and non-Rj, Rj3 or Rj4-genotype cultivars were significantly different. These results suggest that the Rj 2-gene might not only affect the nodulation compatibility between Rj-genotype soybeans and bradyrhizobia, but also the nodulation tendency of the bradyrhizobia.

Original languageEnglish
Pages (from-to)65-72
Number of pages8
JournalSoil Science and Plant Nutrition
Volume55
Issue number1
DOIs
Publication statusPublished - Feb 1 2009

Fingerprint

Andosol
nodulation
Bradyrhizobiaceae
soybean
genotype
cultivar
soybeans
cultivars
restriction fragment length polymorphism
Bradyrhizobium
diversity index
USDA
polymerase chain reaction
internal transcribed spacers
Andosols
polymorphism
gene
genes

All Science Journal Classification (ASJC) codes

  • Soil Science
  • Plant Science

Cite this

Estimation of nodulation tendency among Rj-genotype soybeans using the bradyrhizobial community isolated from an Andosol. / Minami, Mai; Yamakawa, Takeo; Yamamoto, Akihiro; Akao, Shoichiro; Saeki, Yuichi.

In: Soil Science and Plant Nutrition, Vol. 55, No. 1, 01.02.2009, p. 65-72.

Research output: Contribution to journalArticle

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