Estimation of self-incompatibility genotypes of Citrus cultivars with Got-3 allozyme markers

Binh Xuan Ngo, Jung Hee Kim, Akira Wakana, Shiro Isshiki, Tomoyo Mori

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Self-incompatibility (SI) in Citrus is known in cultivars and plants of pummelo, pummelo relative, and mandarin; however, the detailed mechanism of self-incompatibility and the allelic diversity of the SI gene (S) are not known because of the presence of barriers such as nucellar embryony, male sterility, and a long juvenile phase. Thus, there is little information on S genotypes of Citrus cultivars at present. In this study, the S genotypes of Citrus cultivars were estimated with the aid of allozymes produced by a glutamate oxaloacetate transaminase isozyme gene (Got-3), which appeared to link to the S gene. Of twenty-two F 1 progenies from eleven crosses and their reciprocal crosses with eight monoembryonic SI cultivars, including 'Banpeiyu' pummelo, three F 1 progenies from three crosses and three from reciprocal crosses showed segregation distortion for the Got-3 gene, suggesting the presence of the same S alleles in the cultivars used for the reciprocal crosses. Defining the 'Banpeiyu' genotype as S 1S 2, the genotype was estimated as S 1S 3 for 'Tosa Buntan', S 4S 5 for 'Hassaku', S 6S 7 for 'Yuge-hyokan', and S 1S 6 for 'Shishiyuzu'. Further allozyme analyses for segregation distortion in progenies from eight crosses with eight monoembryonic SI cultivars suggested the possibility that 'Hyuganatsu' has S 1 allele, while those from 15 crosses between the eight monoembryonic SI cultivars and five polyembryonic self-compatible cultivars suggested the possibility that 'Rough lemon' has S 1 allele. The estimated genotypes, consisting of two of the nine alleles (S 1 to S 8 and S f) in these Citrus cultivars, may be useful for further estimation and determination of S genotypes in Citrus cultivars and plants. JSHS

Original languageEnglish
Pages (from-to)284-294
Number of pages11
JournalJournal of the Japanese Society for Horticultural Science
Volume80
Issue number3
DOIs
Publication statusPublished - Aug 2 2011

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allozymes
Citrus
genotype
cultivars
reciprocal crosses
alleles
segregation distortion
genes
Citrus jambhiri
male fertility
mandarins
aspartate transaminase
isozymes

All Science Journal Classification (ASJC) codes

  • Horticulture

Cite this

Estimation of self-incompatibility genotypes of Citrus cultivars with Got-3 allozyme markers. / Ngo, Binh Xuan; Kim, Jung Hee; Wakana, Akira; Isshiki, Shiro; Mori, Tomoyo.

In: Journal of the Japanese Society for Horticultural Science, Vol. 80, No. 3, 02.08.2011, p. 284-294.

Research output: Contribution to journalArticle

Ngo, Binh Xuan ; Kim, Jung Hee ; Wakana, Akira ; Isshiki, Shiro ; Mori, Tomoyo. / Estimation of self-incompatibility genotypes of Citrus cultivars with Got-3 allozyme markers. In: Journal of the Japanese Society for Horticultural Science. 2011 ; Vol. 80, No. 3. pp. 284-294.
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abstract = "Self-incompatibility (SI) in Citrus is known in cultivars and plants of pummelo, pummelo relative, and mandarin; however, the detailed mechanism of self-incompatibility and the allelic diversity of the SI gene (S) are not known because of the presence of barriers such as nucellar embryony, male sterility, and a long juvenile phase. Thus, there is little information on S genotypes of Citrus cultivars at present. In this study, the S genotypes of Citrus cultivars were estimated with the aid of allozymes produced by a glutamate oxaloacetate transaminase isozyme gene (Got-3), which appeared to link to the S gene. Of twenty-two F 1 progenies from eleven crosses and their reciprocal crosses with eight monoembryonic SI cultivars, including 'Banpeiyu' pummelo, three F 1 progenies from three crosses and three from reciprocal crosses showed segregation distortion for the Got-3 gene, suggesting the presence of the same S alleles in the cultivars used for the reciprocal crosses. Defining the 'Banpeiyu' genotype as S 1S 2, the genotype was estimated as S 1S 3 for 'Tosa Buntan', S 4S 5 for 'Hassaku', S 6S 7 for 'Yuge-hyokan', and S 1S 6 for 'Shishiyuzu'. Further allozyme analyses for segregation distortion in progenies from eight crosses with eight monoembryonic SI cultivars suggested the possibility that 'Hyuganatsu' has S 1 allele, while those from 15 crosses between the eight monoembryonic SI cultivars and five polyembryonic self-compatible cultivars suggested the possibility that 'Rough lemon' has S 1 allele. The estimated genotypes, consisting of two of the nine alleles (S 1 to S 8 and S f) in these Citrus cultivars, may be useful for further estimation and determination of S genotypes in Citrus cultivars and plants. JSHS",
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